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掃一掃,進入“其他試用”,免費申請LncRNA對照病毒
科維創(chuàng)生物L(fēng)ncRNA的功能研究
A、LncRNA的表達變化-漢恒生物獨有高靈敏度LncRNA檢測技術(shù)(LncPrimerTM)
通過漢恒生物獨有高靈敏度LncRNA檢測引物庫(LncPrimerTM),利用QPCR、FISH技術(shù),在各種細(xì)胞、動物模型中,檢測LncRNA在細(xì)胞和組織水平的分布和變化。
B、Gain of Function(體內(nèi)和體外)
利用科維創(chuàng)生物HanbioTM系列高效表達病毒載體(慢病毒、腺病毒等)全長過表達LncRNA
圖:科維創(chuàng)生物HanbioTM系列-高效過表達慢病毒和腺病毒載體
C、Loss of Function(體內(nèi)和體外)
◆ 合成片段siRNA;
◆ 科維創(chuàng)生物HanbioTM系列高效表達病毒載體(慢病毒、腺病毒等)表達shRNA;
圖:科維創(chuàng)生物HanbioTM系列-高效下調(diào)慢病毒和腺病毒載體
◆ ASO(antisenseoligonucleotide,反義寡核苷酸),主要針對細(xì)胞核內(nèi)的反義RNA起作用。
技術(shù)優(yōu)勢
1.科維創(chuàng)生物擁有由若干LncRNA研究專家組成的專業(yè)LncRNA研究團隊,技術(shù)水平卓越,專注于LncRNA整體服務(wù),可為您提供一體化的LncRNA系統(tǒng)研究方案;
2.科維創(chuàng)生物已成功完成許多LncRNA項目,擁有成熟的LncRNA相關(guān)經(jīng)驗;
3.如需要詳細(xì)的LncRNA課題/實驗指導(dǎo),歡迎登陸科維創(chuàng)官網(wǎng)www.kescience.com與科維創(chuàng)生物的LncRNA專家交流探討更多細(xì)胞功能學(xué)研究領(lǐng)域。直擊以下鏈接通過QQ與我們的技術(shù)工程師一對一交流!
http://wpa.b.qq.com/cgi/wpa.php?ln=1&key=XzgwMDA1Mjg2M18xMjcwNzFfODAwMDUyODYzXzJf
參考文獻
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Nature Genetics 37 (7): 766–70. doi:10.1038/ng1590. PMID 15965474.
[3] Birney E, Stamatoyannopoulos JA, Dutta A, et al. (June 2007). "Identification and analysis of functional elements in 1% of the human genome
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[4] Braidotti G, Baubec T, Pauler F, et al. (2004). "The Air noncoding RNA: an imprinted cis-silencing transcript". Cold Spring Harbor Symposia
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[5] Brosius J (May 2005). "Waste not, want not--transcript excess in multicellular eukaryotes". Trends in Genetics 21 (5): 287–8.
doi:10.1016/j.tig.2005.02.014. PMID 15851065.
[6] Calin GA, Liu CG, Ferracin M, et al. (September 2007). "Ultraconserved regions encoding ncRNAs are altered in human leukemias and
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[7] Costanzi C, Pehrson JR (June 1998). "Histone macroH2A1 is concentrated in the inactive X chromosome of female mammals".
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[8] Derrien, T.; Johnson, R.; Bussotti, G.; Tanzer, A.; Djebali, S.; Tilgner, H.; Guernec, G.; Martin, D.; Merkel, A.; Knowles, D. G.; Lagarde, J.;
Veeravalli, L.; Ruan, X.; Ruan, Y.; Lassmann, T.; Carninci, P.; Brown, J. B.; Lipovich, L.; Gonzalez, J. M.; Thomas, M.; Davis, C. A.;
Shiekhattar, R.; Gingeras, T. R.; Hubbard, T. J.; Notredame, C.; Harrow, J.; Guigó, R. (2012). "The GENCODE v7 catalog of human
long noncoding RNAs: Analysis of their gene structure, evolution, and expression". Genome Research 22 (9): 1775–1789.
doi:10.1101/gr.132159.111.
[9] Faghihi MA, Modarresi F, Khalil AM, et al. (July 2008). "Expression of a noncoding RNA is elevated in Alzheimer's disease and drives rapid
feed-forward regulation of beta-secretase". Nature Medicine 14 (7): 723–30. doi:10.1038/nm1784. PMC 2826895. PMID 18587408.
[10] Feng J, Bi C, Clark BS, Mady R, Shah P, Kohtz JD (June 2006). "The Evf-2 noncoding RNA is transcribed from the Dlx-5/6 ultraconserved
region and functions as a Dlx-2 transcriptional coactivator". Genes & Development 20 (11): 1470–84. doi:10.1101/gad.1416106.
PMC 1475760. PMID 16705037.
[11] Hesman Saey, Tina (17 December 2011). "Missing Lincs". Science News 180 (13): 22–25.Ishii N, Ozaki K, Sato H, et al. (2006).
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