• Species Reactivity

  Mouse

• Specificity

  Detects mouse Sortilin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross?reactivity with recombinant human Sortilin is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Sortilin    
  Gly76-Asn753    
  Accession # Q6PHU5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Simple Western

  10 μg/mL

  See below

• 
  

• Immunohistochemistry

  1-15 μg/mL

  See below

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 1.5-6 μg/mL of this antibody will block 50% of the binding of 200 ng/mL of Recombinant Human beta -NGF (Catalog # ) to immobilized Recombinant Mouse Sortilin (Catalog # ) coated at 4 μg/mL (100 μL/well). At 100 μg/mL, this antibody will block >90% of the binding.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Sortilin

Sortilin (neurotensin receptor 3, glycoprotein 95) is a 95 kDa Type I transmembrane monomeric glycoprotein that is one of five known members of the mammalian vacuolar protein sorting 10p domain (Vps10p-D) family of sorting receptors (1, 2). Mouse preprosortilin is processed by signal sequence cleavage followed by propeptide cleavage at a furin recognition site. The cationic propeptide exhibits pH-dependent high affinity binding that blocks the Sortilin ligand binding site both pre? and post-cleavage (3). The extracellular/luminal sequence comprises the Vps10p domain, including 10 conserved cysteines (10CC) essential for ligand binding (2). The cytoplasmic domain sorting motifs confer all trafficking during synthesis, targeting to lysosomes, endocytosis and Golgi-endosome transport; as little as 10% may be found on the cell surface (4). Mature mouse Sortilin shares 98% amino acid (aa) identity with rat, and 91% aa identity with human and canine sortilin. During murine development, sortilin is mainly expressed in the nervous system (5), where it is a receptor for neuropeptides including neurotensin, nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) (6?9). ProNGF (or the NGF propeptide alone) binds sortilin with a much higher affinity (K_D ~5-8 nM) than does mature NGF (K_D ~90 nM). The complex of sortilin, pro?NGF and the receptor p75^ntr results in endocytosis of proNGF and induction of apoptosis (7). Similar results have been obtained with proBDNF and BDNF (8?9). Sortilin is expressed in other tissues including testis, skeletal muscle and fat (1, 10). It is essential and sufficient for biogenesis of Glut4 storage vesicles necessary for insulin responsiveness in adipocytes (10). Sortilin also binds lipoprotein lipase (11), apoE (2) and RAP (1, 11). Binding is competitive, indicating that although unrelated, targets likely bind the same site.

• References:

1. Petersen, C.M. et al. (1997) J. Biol. Chem. 272:3599.

2. Westergaard, U.B. et al. (2004) J. Biol. Chem. 279:50221. 

3. Petersen, C.M. et al. (1998) EMBO J. 18:595. 

4. Nielsen, M.S. et al. (2001) EMBO J. 20:2180. 

5. Hermans-Borgmeyer, I. et al. (1999) Mol. Brain Res. 65:216.

6. Mazella, J. et al. (1998) J. Biol. Chem. 273:26273.

7. Nykjaer, A et al. (2004) Nature 427:843.

8. Teng, H.K. et al. (2005) J. Neurosci. 25:5455.

9. Chen, Z.-Y. et al. (2004) J. Neurosci. 25:6156.

10. Shi, J and K.V. Kandror (2005) Dev. Cell 9:99.

11. Nielsen, M.S. et al. (1999) J. Biol. Chem. 274:8832.

• Entrez Gene IDs:

  6272 (Human); 20661 (Mouse)

• Alternate Names:

  100 kDa NT receptor; Glycoprotein 95; Gp95; Gp95LDLCQ6; Neurotensin receptor 3; NT3NTR3; Ntr3; SORT1; sortilin 1; Sortilin