• Species Reactivity

  Human

• Specificity

  Detects human IGSF4B/SynCAM3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) IGSF3, 4, 4C, 4D, 8, or recombinant mouse IGSF4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 730014

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human IGSF4B/SynCAM3    
  Pro21-Tyr329    
  Accession # Q8N126

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.2 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IGSF4B/SynCAM3

IGSF4B (immunoglobulin superfamily member 4B), also called CADM3, TSLL1, Necl?1 and SynCAM3, is a neural tissue-specific member of the nectin-like family of immunoglobulin superfamily (1, 2). It is a 48?50 kDa type I transmembrane (TM) glycoprotein that is concentrated at non-junctional contact sites of neuronal axons and glial processes (2, 3). In myelinated peripheral nerve fibers, IGSF4B is concentrated at nodes of Ranvier in contact sites of Schwann cells (2). The 398 amino acid (aa) human IGSF4B contains a 24 aa signal sequence, a 306 aa extracellular domain (ECD), a 21 aa transmembrane domain and a 47 aa cytoplasmic domain. The ECD contains an N-terminal V-type Ig-like domain that is responsible for Ca⁺⁺-independent homophilic and heterophilic interactions with Nectin-1, Nectin-3 or Necl?2 (IGSF-4) in trans. It also contains two C2-type Ig-like domains that are responsible for Ca⁺⁺-independent homophilic dimerization in cis that is thought to precede trans interaction (2, 4, 5). The cytoplasmic domain binds members of the MAGUK guanylate kinase subfamily, such as Dlg3, Pals2 and CASK (2). These activities are thought to play roles in adhesion and architecture at the synapse (2). We have also shown that IGSF4B promotes in vitro outgrowth of cortical neurons. Of two known splice variants, one shows an insertion of 34 aa near the mature N-terminus and probably represents a 60 kDa form. A smaller variant shows deletions of aa 185?231, and 281?398, which includes the first C2-type Ig-like domain and the TM segment. The IGSF4B ECD is highly conserved, sharing 95?96% aa identity between human mouse, rat, canine and bovine sequences. The ECD of IGSF4A, B, C and D proteins share 35?50% aa identity.

• References:

1. Fukuhara, H. et al. (2001) Oncogene 20:5401.

2. Kakunaga, S. et al. (2005) J. Cell Sci. 118:1267.

3. Fukami, T. et al. (2003) Gene 323:11.

4. Shingai, T. et al. (2003) J. Biol. Chem. 278:35421

5. Dong, X. et al. (2006) J. Biol. Chem. 281:10610.

• Long Name:

  Immunoglobulin Superfamily, Member 4B

• Entrez Gene IDs:

  57863 (Human); 94332 (Mouse); 360882 (Rat)

• Alternate Names:

  BIgR; Brain immunoglobulin receptor; CADM3; cell adhesion molecule 3; IGSF4B; IGSF4BSynCAM3; Immunoglobulin superfamily member 4B; member 4B; Necl-1; NECL1TSLC1-like protein 1; Nectin-like protein 1; Synaptic cell adhesion molecule 3; SynCAM3; TSLC1-like 1; TSLL1; TSLL1SYNCAM3