• Species Reactivity

  Human

• Specificity

  Detects human Claudin?8. Stains human Claudin-8 transfectants but not irrelevant transfectants.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 474210

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  HEK293 human embryonic kidney cell line transfected with human Claudin-8    
  Met1-Val225 (Ser151Pro)    
  Accession # P56748

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Claudin-8

Claudin-8 is a 225 amino acid (aa), 25 kDa four-transmembrane protein in the Claudin family of tight junction proteins. It is selectively expressed in tight junctions in the kidney thin descending limb, distal tubule and collecting duct, but is also found in liver and intestinal tight junctions. It is thought to function as an ion barrier protein. The extracellular portions of human Claudin-8 share 86% and 89% aa identity with corresponding portions of mouse and rat Claudin-8, respectively.

• Entrez Gene IDs:

  9073 (Human); 54420 (Mouse); 304124 (Rat)

• Alternate Names:

  claudin 8; Claudin8; Claudin-8; CLDN8; human CLDN8 gene for claudin-810claudin-8