• Species Reactivity

  Human

• Specificity

  Detects human beta -Arrestin 1 in direct ELISAs and Western blots.

• Source

  Monoclonal Rat IgG    _2A Clone # 425817

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human beta -Arrestin 1    
  Met1-Arg418    
  Accession # P49407

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

• Immunohistochemistry

  8-25 μg/mL

  Immersion fixed paraffin-embedded sections of human colon cancer tissue

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: beta-Arrestin 1

The Arrestin family consists of four members: Arrestin 1 (visual Arrestin), Arrestin 2 ( beta -Arrestin 1), Arrestin 3 ( beta -Arrestin 2), and Arrestin 4 (cone Arrestin). While visual and cone Arrestins are found almost exclusively in the retina, beta -Arrestins 1 and 2 are ubiquitously expressed, and were initially described as negative regulators of G protein-coupled receptor (GPCR) signaling. More recently, beta -Arrestins have been determined to serve as scaffolds for various signaling pathways, including the MAPK cascades activating ERK2, p38 alpha, and JNK3. These beta -Arrestin scaffolds tie together the appropriate kinases in series, forming a discreet signaling module that localizes components to specific subcellular environments and facilitates greater kinase activation.

• Entrez Gene IDs:

  408 (Human); 109689 (Mouse); 25387 (Rat)

• Alternate Names:

  ARB1; ARR1; ARRB1; arrestin 2; Arrestin beta-1; arrestin, beta 1; betaArrestin 1; beta-Arrestin 1; beta-arrestin-1