• Species Reactivity

  Human

• Specificity

  Detects human DTX1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 100% cross-reactivity with recombinant human DTX4 is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 730612

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human DTX1/DTX4    
  Met1-Phe147    
  Accession # Q86Y01

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

• CyTOF-ready

  Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

• Intracellular Staining by Flow Cytometry

  0.25 μg/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DTX1/DTX4

Deltex-1 (DTX1) is an approximately 75 kDa cytoplasmic and nuclear ubiquitin ligase that interacts with the Notch-1 intracellular domain and regulates Notch-induced gene transcription. It contains two WWE domains (aa 14-94 and aa 95-171) and one RING-type zinc finger (aa 411-472). Within aa 1-147, human Deltex-1 shares 97% aa sequence identity with mouse and rat Deltex-1.

• Long Name:

  Deltex 1/4

• Alternate Names:

  DTX1/DTX4