• Species Reactivity

  Mouse

• Specificity

  Detects mouse Kirrel2/NEPH3 in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 40% cross?reactivity with recombinant human Kirrel2 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse Kirrel2/NEPH3    
  Ser19-Asp501    
  Accession # Q7TSU7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse Kirrel2 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Kirrel2/NEPH3

Kirrel2 (kin of irregular chiasm-1-like 2), also known as filtrin, NEPH3 (nephrin-like 3) or NLG1 (nephrin-like gene 1), is a 107 kDa type I transmembrane adhesion protein belonging to the immunoglobulin superfamily (1, 2). Kirrel2 is one of several proteins expressed both in pancreatic islet beta cells and kidney glomerulus, and is also found in brain and lymph nodes (1?9). Mouse Kirrel2 cDNA encodes a 700 amino acid (aa) protein that contains a 16 aa signal sequence, a 491 aa extracellular domain with five C2-type Ig-like domains, a 21 aa transmembrane sequence and a 172 aa intracellular domain with a nephrin-like, conserved podocin binding site (mouse aa 589?598) (4, 5). An extracellular RGD sequence allows for cell attachment (4). The extracellular domain of mouse Kirrel2 shares 96%, 86%, 85% and 85% aa identity with rat, human, equine and bovine Kirrel2, respectively. It shares 35?37% aa identity with other mouse Kirrel proteins. One mouse isoform that has been described is missing the transmembrane domain (aa 501?533), while another encodes only portions of Ig domains 1?4 (5). Human Kirrel2 is downregulated in glomeruli of patients with proteinuria, suggesting an involvement in the maintenance of the glomerular filtration barrier (6). Autoantibodies to Kirrel2 have been identified in a subset of humans with type I diabetes, indicating its involvement with islet beta cell function (7). In the brain, Kirrel2 is present in olfactory neurons; but only when they are active; homophilic adhesion of Kirrel2 is postulated to help correlate nerves identifying the same odorant (8). It is also a marker for mouse early postmitotic neural precursors in the ventricular zone of the developing mouse spinal cord (9).

• References:

1. Sun C. et al. (2003) Genomics 82:130.

2. Ihalmo, P. et al. (2003) Biochem. Biophys. Res. Commun. 300:364.

3. Rinta-Valkama, J. et al. (2007) Mol. Cell. Biochem. 294:117.

4. Sellin, L. et al. (2003) FASEB J. 17:115.

5. SwissProt Accession # Q7TSU7.

6. Ihalmo, P. et al. (2007) Nephrol. Dial. Transplant 22:1903.

7. Rinta-Valkama, J. et al. (2007) Diabetes Metab. Res. Rev. 23:119.

8. Serizawa, S. et al. (2006) Cell 127:1057.

9. Minaki, Y. et al. (2005) Neurosci. Res. 52:250.

• Long Name:

  Kin of Irregular Chiasm-like 2, Splice Variant A

• Entrez Gene IDs:

  84063 (Human); 243911 (Mouse)

• Alternate Names:

  DKFZp564A1164; Filtrin; FILTRINkin of IRRE-like protein 2; kin of IRRE like 2 (Drosophila); Kin of irregular chiasm-like protein 2; Kirrel2; MGC15718; NEPH3; NEPH3Nephrin-like protein 3; nephrin-like 3; nephrin-like gene 1; NLG1; NLG1kin of irregular chiasm-like 2