• Purity

  >98%, by HPLC

• Activity

  Add Recombinant Human NEDD8 Vinyl Sulfone to     in vitro assays to inhibit NEDD8-specific isopeptidases. Reaction conditions will need to be optimized for each specific application. We recommend an initial Recombinant Human NEDD8 Vinyl Sulfone concentration of 1-5 μM.

• Source

  E. coli-derived

• Accession #

Background: NEDD8

NEDD8 (Neural precursor cell-expressed developmentally down-regulated gene 8; also known as Rub1) is a 6-8 kDa member of the ubiquitin family of proteins. It is expressed in striated muscle, and via its C-terminal Gly, forms covalent bonds with cullin family proteins. This conjugation generates ubiquitin ligase activity that regulates cell cycle proteins. Human pro-NEDD8 is 81 amino acids (aa) in length. It contains one ubiquitin-like domain (aa 1-65), and a 4-5 aa C-terminal propeptide that is cleaved to expose a C-terminal Gly residue that is used to generate a Gly-Lys intermolecular bond. Mature human NEDD8 shows 100% aa identity to NEDD8 from both mouse and canine.

• Long Name:

  Neural Precursor Cell Expressed Developmentally Down-Regulated Protein 8

• Entrez Gene IDs:

  4738 (Human); 18002 (Mouse); 25490 (Rat)

• Alternate Names:

  FLJ43224; MGC104393; MGC125896; MGC125897; NEDD8; NEDD-8; Neddylin; Neural precursor cell expressed developmentally down-regulated protein 8; neural precursor cell expressed, developmentally down-regulated 8; Rub1; Ubiquitin-like protein Nedd8