• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Detects human, mouse, and rat STAT5b in Western blots.

• Source

  Recombinant Monoclonal Rabbit IgG Clone # 1032E

• Purification

  Protein A or G purified from cell culture supernatant

• Immunogen

  Human STAT5b synthetic peptide aa 777-787    
  Accession # P51692

• Formulation

  Supplied as a solution in PBS containing BSA, Glycerol and Sodium Azide. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1:1000 dilution

  See below

• 
  

• Simple Western

  1:100 dilution

  See below

• 
  

• Immunocytochemistry

  1:100 dilution

  See below

• 
  

• Knockout Validated

  STAT5b is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in STAT5b knockout HeLa cell line.

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C, as supplied.

• 1 month, 2 to 8 °C under sterile conditions after opening.

• 6 months, -20 to -70 °C under sterile conditions after opening.

Background: STAT5b

Signal Transduction and Activator of Transcription 5 (STAT5) is a member of the Jak/STAT signal transduction pathway and is activated by a variety of cytokines (IL?22, IL-6, IFN-alpha ). STAT5 has two isoforms (A and B) that share 93% amino acid identity and bind the DNA consensus site TTCN₃GAA. STAT5 mediates cytokine signaling by acting as a signal transducer in the cytoplasm and, upon phosphorylation, translocates to the nucleus and activates transcription of specific genes. STAT5 is involved in a wide array of biological processes ranging from regulating apoptosis to adult mammary gland proliferation, differentiation, and survival.

• Long Name:

  Signal Transducer and Activator of Transcription 5b

• Entrez Gene IDs:

  6777 (Human); 20851 (Mouse)

• Alternate Names:

  signal transducer and activator of transcription 5B; STAT5; STAT5b; transcription factor STAT5B