• Species Reactivity

  Mouse

• Specificity

  Detects mouse JAM-C in direct ELISAs and Western blots. In Western blots, approximately 5% cross-reactivity with recombinant human JAM?C and less than 2% cross-reactivity with recombinant mouse (rm) JAM-A and rmJAM-B is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse JAM-C    
  Val32-Asn241    
  Accession # Q9D8B7

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse JAM-C Fc Chimera (Catalog # )

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

• Blockade of Receptor-ligand Interaction

  In a functional ELISA, 3-15 μg/mL of this antibody will block 50% of the binding of 10 ng/mL of biotinylated Recombinant Mouse VEJAM to immobilized Recombinant Mouse JAM-C (Catalog # ) coated at 2 μg/mL (100 μL/well). At 30 μg/mL, this antibody will block >90% of the binding.

• 
  

• Immunocytochemistry

  5-15 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: JAM-C

The family of junctional adhesion molecules (JAM), comprised of at least three members, are type I transmembrane receptors belonging to the immunoglobulin (Ig) superfamily (1, 2). These proteins are localized in the tight junctions between endothelial cells or epithelial cells. Some family members are also found on blood leukocytes and platelets. Mouse JAM-C cDNA predicts a 310 amino acid (aa) residue  precursor protein with a putative 31 aa signal peptide, a 210 aa extracellular region containing two Ig domains, an 18 aa transmembrane domain and a 51 aa cytoplasmic domain containing a PDZ-binding motif and a PKC phosphorylation site (3). Mouse JAM-C shares 86% aa sequence identity with its human homologue. It also shares approximately 31% and 35% aa sequence homology with mouse JAM-A and JAM-B, respectively (2). Mouse JAM-C is highly expressed during embryogenesis. In adult tissues, mouse JAM-C is restricted to endothelial cells, lymph endothelial cells in the kidney, lymph node and Peyer’s patches where the protein can be localized to the high endothelial venules (3). Although human JAM-C is expressed on human platelets and a subset of leukocytes, mouse JAM-C expression was not detected on any mouse lymphocytes (4). In contrast to human JAM-C which show weak homotypic interactions, mouse JAM-C was reported to exhibit homotypic interactions (3). Mouse JAM-C has also been shown to have heterotypic interaction with JAM-B. It is likely that mouse JAM-C may play a role in lymphocyte transendothelial migration (4).

The nomenclature used for the JAM family proteins is confusing. VE-JAM has been referred to in the literature variously as JAM-B or JAM-C. Until further clarification, R&D Systems has adopted the nomenclature where both mouse and human VE-JAM are referred to as JAM-B.

• References:

1. Chavakis, T. et al. (2003) Thromb. Haemost. 89:13.

2. Aurrand-Lions, M. et al. (2001) Blood 98:3699.

3. Aurrand-Lions, M. et al. (2001) J. Biol. Chem. 276:2733.

4. Johnson-Leger, C. et al. (2002) Blood 100:25793.

• Long Name:

  Junctional Adhesion Molecule C

• Entrez Gene IDs:

  83700 (Human); 83964 (Mouse)

• Alternate Names:

  CD323; JAM-2; JAM3; JAMC; JAM-C; JAM-CFLJ14529; junctional adhesion molecule 3JAM-3; junctional adhesion molecule C