• Species Reactivity

  Mouse

• Specificity

  Detects mouse IL-20 in direct ELISAs and the Western blots. In direct ELISAs and Western blots, approximately 10% cross-reactivity with recombinant human IL-20 is observed.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant mouse IL?20    
  Leu25-Leu176    
  Accession # Q9JKV9

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Endotoxin Level

  <0.10 EU per 1 μg of the antibody by the LAL method.  

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.1 μg/mL

  Recombinant Mouse IL?20 (Catalog # )

• 
  

• Neutralization

  Measured by its ability to neutralize IL?20-induced proliferation in the BaF3 mouse pro?B cell line co-transfected with human IL?20 R alpha and IL?20 R beta. The Neutralization Dose (ND    ₅₀) is typically 0.2-0.6 μg/mL in the presence of 10 ng/mL Recombinant Mouse IL?20.

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-20

Mouse Interleukin 20 (IL-20) was identified by searching sequence databases for translated sequences with a signal sequence and amphipathic helices found in helical cytokines. Based on the human molecule, mouse IL-20 was discovered in a skin library. Mouse IL-20 is synthesized as a 176 amino acid (aa) precursor that contains a 24 aa signal sequence and a 152 aa mature segment. There are no N-linked glycosylation sites and it is doubtful that the native molecule is glycosylated. Although IL-20 is a distant member of the IL-10 family, it functions as a monomer. IL-20 shares less than 40% aa sequence identity with other IL-10 family members. Mouse and human IL-20 are 77% aa identical in the mature segment. IL-20 production has been found in skin and trachea. In particular, activated keratinocytes and, possibly, monocytes are reported to express IL-20. There are two heterodimeric receptor complexes for IL-20. The first complex is composed of IL-20 R alpha and IL?20 R beta. The second complex is composed of IL-22 R and IL?20 R beta. Whereas the IL?22 R/IL?20 R beta  complex is shared with IL-24/mda-7, the IL?20 R alpha /IL?20 R beta complex is shared with both IL-19 and IL-24. Little is known about the function of IL-20. It is reported to induce the proliferation of multipotential hematopoietic progenitor cells, direct the differentiation and expansion of keratinocytes, and promote the release of proinflammatory mediators in keratinocytes and other IL-20 receptor expressing cells (1?6).

• References:

1. Blumberg, H. et al. (2001) Cell 104:9.

2. Liu, L. et al. (2003) Blood 102:3206.

3. Rich, B.E. and T.S. Kupper (2001) Curr. Biol. 11:R531.

4. Pestka, S. et al. (2004) Annu. Rev. Immunol. 22:929.

5. Dumoutier, L. (2001) J. Immunol. 167:3545. 

6. Romer, J. (2003) J. Invest. Dermatol. 121:1306.

• Long Name:

  Interleukin 20

• Entrez Gene IDs:

  50604 (Human); 58181 (Mouse)

• Alternate Names:

  Cytokine Zcyto10; IL10D; IL20; IL-20; IL-20ZCYTO10four alpha helix cytokine; interleukin 20; interleukin-20; MGC96907