• Species Reactivity

  Human

• Specificity

  Detects human Fucosyltransferase 8/FUT8 in direct ELISAs and Western blots.    
   

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Fucosyltransferase 8/FUT8     
  Asp32-Lys575    
  Accession # Q9BYC5

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Simple Western

  10 μg/mL

  See below

• 
  

• Immunohistochemistry

  5-15 μg/mL

  See below

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Human Fucosyltransferase 8/FUT8,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Fucosyltransferase 8/FUT8

FUT8 (Fucosyltransferase 8; also alpha 1,6 Fucosyltransferase) is a 60?65 kDa member of the glycosyltransferase 23 family of enzymes. It catalyzes the addition of fucose to Asn-linked GlcNAc via an alpha 1,6 linkage. FUT8 is widely expressed, and the effects of its fucosylation vary. A lack of fucosylation on IgG potentiates ADCC activity, while an absence of fucose on the EGF R and alpha 3 beta 1 integrin decreases their activity. Human FUT8 is a type II transmembrane protein 575 amino acids (aa) in length. It contains a short cytoplasmic region (aa 1?9) plus an extended luminal domain (aa 31?575) that possesses one SH3 homology domain (aa 502?563). There are at least four potential splice variants. There is an alternate start site at Met479, a 14 aa substitution for aa 1?420, a 32 aa substitution for aa 1?161, and a two aa substitution for aa 444?575. Over aa 32?575, human FUT8 shares 97% aa identity with mouse FUT8.

• Entrez Gene IDs:

  2530 (Human); 53618 (Mouse); 432392 (Rat)

• Alternate Names:

  alpha 1,6 Fucosyltransferase; alpha-(1,6)-fucosyltransferase; alpha1-6FucT; EC 2.4.1.68; fucosyltransferase 8 (alpha (1,6) fucosyltransferase); Fucosyltransferase 8; FUT8; GDP-fucose--glycoprotein fucosyltransferase; GDP-L-Fuc:N-acetyl-beta-D-glucosaminide alpha1,6-fucosyltransferase; Glycoprotein 6-alpha-L-fucosyltransferase; MGC26465