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詳細說明
概述
產(chǎn)品名稱Anti-NNF1R抗體[EPR17298]
描述
兔單克隆抗體[EPR17298] to NNF1R
經(jīng)測試應用IP,WB
種屬反應性
與反應: Mouse, Rat, Human
免疫原
Recombinant full length protein within Human NNF1R aa 1 to the C-terminus. The exact sequence is proprietary.
Database link: Q6P1K2Run BLAST with
Run BLAST with 
陽性對照
HepG2 whole cell lysate Jurkat whole cell lysate HEK293 whole cell lysate, HeLa whole cell lysate, Human fetal brain tissue lysate, Human fetal kidney tissue lysate, C6 whole cell lysate, Raw264.7 whole cell lysate, NIH/3T3 whole cell lysate,
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR17298
同種型IgG
研究領(lǐng)域
Epigenetics and Nuclear Signaling
Cell cycle
Chromosome Structure
Centromere
Cell Biology
Cell Cycle
Cell Division
Spindle
Anti-NNF1R antibody [EPR17298] 圖像
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB1.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : HepG2 (Human liver hepatocellular carcinoma) whole cell lysate
Lane 2 : Jurkat (Human T cell leukemia cells from peripheral blood) whole cell lysate
Lane 3 : HEK293 (Human epithelial cells from embryonic kidney) whole cell lysate
Lane 4 : HeLa (Human epithelial cells from cervix adenocarcinoma ) whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB2.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/5000 dilution
Lane 1 : Human fetal brain tissue lysate
Lane 2 : Human fetal kidney tissue lysate
Lysates/proteins at 10 μg per lane.
Secondary
Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking and diluting buffer was 5% NFDM /TBST.
![Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199531WB3.jpg)
Western blot - Anti-NNF1R antibody [EPR17298] (ab199531)
All lanes : Anti-NNF1R antibody [EPR17298] (ab199531) at 1/1000 dilution
Lane 1 : C6 (Rat glial tumor cells) whole cell lysate
Lane 2 : Raw264.7 (Mouse macrophage cells transformed with Abelson murine leukemia virus) whole cell lysate
Lane 3 : NIH/3T3 (Mouse embryo fibroblast cells) whole cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
developed using the ECL technique
Predicted band size : 23 kDa
Observed band size : 26 kDa (why is the actual band size different from the predicted?)
Exposure time : 30 secondsBlocking and diluting buffer was 5% NFDM /TBST.
![Immunoprecipitation - Anti-NNF1R antibody [EPR17298] (ab199531)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab1995311IP.jpg)
Immunoprecipitation - Anti-NNF1R antibody [EPR17298] (ab199531)
NNF1R was immunoprecipitated from 1mg of HeLa (Human epithelial cells from cervix adenocarcinoma) whole cell extract with ab179466 at 1/80 (or 5 μg). Western blot was performed using ab199531 at 1/1000. Anti-Rabbit IgG (HRP), specific to the non-reduced form of IgG, was used as secondary antibody at 1/1500 dilution.
Lane 1: HeLa whole cell extract at 10ug (input)
Lane 2: HeLa whole cell extract NNF1R immunoprecipitate
Lane 3: Rabbit monoclonal IgG (ab172730) instead of ab199531 in HeLa whole cell extract immunoprecipitation.
Blocking and dilution buffer and concentration: 5% NFDM/TBST.
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