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Anti-nmt55 / p54nrb antibody [EPR5270] 10μl 微信掃碼
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產品介紹

基本參數(shù)

詳細說明

概述

產品名稱Anti-nmt55 / p54nrb抗體[EPR5270]
描述
兔單克隆抗體[EPR5270] to nmt55 / p54nrb
經測試應用WB,ICC/IF,IHC-P
種屬反應性
與反應: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) corresponding to Human nmt55/ p54nrb (C terminal).
Database link: Q15233
陽性對照

WB: PC3, MOLT4, HeLa, MCF7, A673, RAW 264.7, PC12 and NIH/3T3 cell lysates. IHC-P: Human kidney and bladder carcinoma tissues.

常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
We are constantly working hard to ensure we provide our customers with best in class antibodies. As a result of this work we are pleased to now offer this antibody in purified format. We are in the process of updating our datasheets. The purified format is designated ‘PUR’ on our product labels. If you have any questions regarding this update, please contact our Scientific Support team.
Produced using Abcam’s RabMAb^? technology. RabMAb^? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
Alternative versions available:
Anti-nmt55 / p54nrb antibody (Alexa Fluor^? 647) [EPR5270] (ab207375)

Anti-nmt55 / p54nrb antibody (Alexa Fluor? 488) [EPR5270] (ab208404)

性能

形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C.
解離常數(shù)（K_D） K_D = 2.20 x 10 ^-12 M Learn more about K_D
存儲溶液pH: 7.40
Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 0.05% BSA, 40% Glycerol
純度Protein A purified
克隆單克隆
克隆編號EPR5270
同種型IgG
研究領域

Epigenetics and Nuclear Signaling
DNA / RNA
RNA Processing
Splicing

Anti-nmt55 / p54nrb antibody [EPR5270] 圖像

Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution (purified) + PC-12 cell lysate at 20 μg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size : 54 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution (purified) + RAW264.7 cell lysate at 20 μg

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size : 54 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
All lanes : Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/10000 dilution (purified)

Lane 1 : Molt-4 cell lysate
Lane 2 : A673 cell lysate

Lysates/proteins at 20 μg per lane.

Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/10000 dilution (Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated)

Predicted band size : 54 kDa

Blocking buffer and concentration: 5% NFDM/TBST.
Diluting buffer and concentration: 5% NFDM /TBST.
Western blot - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
All lanes : Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574) at 1/1000 dilution (unpurified)

Lane 1 : PC3 cell lysate
Lane 2 : MOLT4 cell lysate
Lane 3 : HeLa cell lysate
Lane 4 : MCF7 cell lysate
Lane 5 : A673 cell lysate
Lane 6 : RAW 264.7 cell lysate
Lane 7 : PC12 cell lysate
Lane 8 : NIH/3T3 cell lysate

Lysates/proteins at 10 μg per lane.

Secondary
Standard HRP labelled goat anti-rabbit at 1/2000 dilution

Predicted band size : 54 kDa
Observed band size : 54 kDa
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of rat kidney tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of mouse liver tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human prostate tissue labelling nmt55/p54nrb with purified ab133574 at 1/500. Heat mediated antigen retrieval was performed using Tris/EDTA buffer pH 9. ab97051, a goat anti-rabbit IgG H&L (HRP) was used as the secondary antibody (1/500). Negative control using PBS instead of primary antibody. Counterstained with hematoxylin.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human bladder carcinoma tissue labelling nmt55 / p54nrb using unpurified ab133574 at a 1/250 dilution.
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)

Immunohistochemical analysis of formalin-fixed, paraffin-embedded Human kidney tissue labelling nmt55 /p54nrb using unpurified ab133574 at a 1/250 dilution.
Immunocytochemistry/ Immunofluorescence - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)

Immunocytochemistry/Immunofluorescence analysis of Molt-4 (human acute lymphoblastic leukemia) cells labelling nmt55/p54nrb with purified ab133574 at 1/1500. Cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100. ab150077, an Alexa Fluor^? 488-conjugated goat anti-rabbit IgG (1/500) was used as the secondary antibody. DAPI (blue) was used as the nuclear counterstain. ab7291, a mouse anti-tubulin (1/1000) and ab150120, an Alexa Fluor^? 594-conjugated goat anti-mouse IgG (1/1000) were also used.
Control 1: primary antibody (1/100) and secondary antibody, ab150120, an Alexa Fluor^? 594-conjugated goat anti-mouse IgG (1/500).
Control 2: ab7291 (1/1000) and secondary antibody, ab150077, an Alexa Fluor^? 488-conjugated goat anti-rabbit IgG (1/500).
Other - Anti-nmt55 / p54nrb antibody [EPR5270] (ab133574)
Equilibrium disassociation constant (K_D)
Learn more about K_D

Click here to learn more about K_D

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