生物來源

human blood

種族

Australian Caucasian

性別

male

生長模式

Suspension

核型

Not specified

形態(tài)學(xué)

Lymphoblastoid

應(yīng)用

cell culture | mammalian: suitable

運輸

dry ice

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相關(guān)類別

Mammalian Cell Lines

一般描述

Consanguineous: No

細胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC).  HLA-Typing Data is available for this cell line.

DNA圖譜分析

A: 0301 2402
Cw: 501
B: 1801
HLA DRA: 101
HLA DRB1: 301
HLA DRB3: 202
HLA DQAP: 4.1
HLA DQA1: 501
HLA DQB1: 201
HLA DPA1: 1
HLA DPB1: 301

血清學(xué)分析

HLA A: 3 24
HLA A ief: 3.2 24.1
HLA C: 5
HLA B: 18
HLA B ief: 18.1
Bw4/bw6: 6
HLA Dw: 3
HLA DR: 3
HLA Dw2: 25
HLA DR52: 52
HLA DQ: 2
HLA DP: 3

培養(yǎng)基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442).  Once a growing culture is established the FBS concentration can be reduced to 10%.

傳代培養(yǎng)常規(guī)

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C.  Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

免責(zé)聲明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.