等級(jí)

for molecular biology

形式

buffered aqueous solution

運(yùn)輸

dry ice

儲(chǔ)存溫度

?20°C

相關(guān)類別

Molecular Cloning & Protein Expression

一般描述

pT7-FLAG^?-2 is a 4.8 kb E . coli expression vector used for cloning and cytoplasmic expression of a properly inserted open reading frame as a N-terminal Met-FLAG fusion protein containing the FLAG^? epitope (DYKDDDDK). The promoter region of the very strong phage T7 promoter  drives transcription of ORFFLAG fusion constructs. This vector requires the use of E. coli cells containing a source of the T7 polymerase, such as BL21(DE3)T1R cells, Catalog Number B2935.

組分

pT7-FLAG^?-1 Expression Vector (P6742) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.
pT7-FLAG^?-1-BAP Control Vector (P6992) is supplied as 0.5 mg/ml in 10 mM Tris-HCl (pH 8.0) with 1 mM EDTA.

法律信息

FLAG is a registered trademark of Sigma-Aldrich Co. LLC

pT7-FLAG is a trademark of Sigma-Aldrich Co. LLC