“英拜助您實(shí)驗(yàn)加速”
Apoptosis miScript miRNA PCR Array細(xì)胞凋亡miRNA PCR芯片

 Apoptosis miScript miRNA PCR Array細(xì)胞凋亡miRNA PCR芯片

 

ProductSpeciesTechnologyCat. No.Apoptosis miScript miRNA PCR ArrayHumanmiRNA ExpressionMIHS-114ZApoptosis miScript miRNA PCR ArrayMousemiRNA ExpressionMIMM-114ZApoptosis miScript miRNA PCR ArrayRatmiRNA ExpressionMIRN-114Z

The Apoptosis miScript miRNA PCR Array profiles the expression of 84 miRNAs that regulate programmed cell death by inhibiting pro-apoptotic and anti-apoptotic gene expression. This array provides researchers with a convenient way to quickly analyze the miRNAs most relevant to apoptosis. Apoptosis plays a critical role in normal biological processes requiring cell removal including differentiation, development, and homeostasis. Stress responses (such as heat shock, ischemia, unfolded proteins, and viral infection) cause badly damaged cells to undergo apoptosis. In cell culture, growth factor withdrawal and many known experimental compounds have a similar effect. An acquired defect in apoptosis activation often leads to uncontrolled cell growth, oncogenesis, and cancer. Ligand-bound tumor necrosis factor (TNF) receptors initiate apoptosis by recruiting FADD and other death domain adaptor proteins that then recruit and activate caspases. Environmental stresses trigger BCL2 protein oligomerization and insertion into the mitochondrial membrane, releasing APAF1 and other CARD family members that also oligomerize to recruit and activate caspases. Caspases promote a proteolytic cascade that degrades cellular protein targets, while the IAP protein family directly inhibits caspases. Besides these post-translational events, miRNAs provide another mechanism of regulating apoptosis by attenuating gene expression. This array represents miRNAs experimentally verified to regulate many pro- and anti-apoptotic genes and to stimulate or attenuate apoptosis upon their overexpression or inhibiton. Monitoring their expression helps determine the mechanisms behind programmed cell death in any model system and the propensity of a cell type to undergo apoptosis normally. A set of controls present on each array enables data analysis using the ΔΔCT method of relative quantification, assessment of reverse transcription performance, and assessment of PCR performance. Using SYBR? Green real-time PCR, the expression of a focused panel of miRNAs related to apoptosis can be easily and reliably analyzed with this miScript miRNA PCR Array.
miScript miRNA PCR Arrays are intended for molecular biology applications. This product is not intended for the diagnosis, prevention or treatment of a disease
細(xì)胞凋亡miRNA PCR芯片檢測(cè)84個(gè)通過(guò)抑制凋亡激活和抗凋亡基因來(lái)調(diào)節(jié)細(xì)胞程序性死亡的microRNA的表達(dá)。這個(gè)芯片為研究人員提供了一個(gè)方便的方法來(lái)快速分析與細(xì)胞凋亡相關(guān)的microRNA。凋亡細(xì)胞在正常生物過(guò)程中起著至關(guān)重要的作用，包括分化、發(fā)育和內(nèi)穩(wěn)態(tài)的調(diào)節(jié)。應(yīng)激反應(yīng)(如熱休克、缺血,展開(kāi)的蛋白質(zhì),和病毒感染)引起嚴(yán)重受損的細(xì)胞發(fā)生凋亡。在細(xì)胞培養(yǎng)中生長(zhǎng)因子消退和許多已知的實(shí)驗(yàn)化合物也有相似的效果。后天的細(xì)胞凋亡激活缺陷往往導(dǎo)致不可控的細(xì)胞生長(zhǎng),腫瘤形成,和癌癥。配體腫瘤壞死因子(TNF)受體啟動(dòng)細(xì)胞凋亡通過(guò)招募FADD和其他死亡結(jié)構(gòu)域接頭蛋白,然后招募和激活凋亡蛋白酶。環(huán)境壓力引發(fā)BCL2蛋白質(zhì)的寡聚化和插入到線粒體膜,釋放APAF1和其他寡聚化的CARD家族成員，來(lái)招募和激活凋亡蛋白酶。凋亡蛋白酶促進(jìn)蛋白質(zhì)級(jí)聯(lián)水解,來(lái)降解細(xì)胞蛋白質(zhì),而IAP蛋白質(zhì)家族直接抑制凋亡蛋白酶。除了這些翻譯后調(diào)控,microRNA提供另一種衰減基因表達(dá)來(lái)調(diào)節(jié)細(xì)胞凋亡的機(jī)制。這個(gè)芯片的microRNA在實(shí)驗(yàn)上已證實(shí)，來(lái)調(diào)控許多促進(jìn)和抑制凋亡的基因，通過(guò)過(guò)表達(dá)或抑制來(lái)促進(jìn)或減弱凋亡。檢測(cè)他們的表達(dá)有助于確定細(xì)胞程序性死亡背后的機(jī)制在任何模型系統(tǒng)和經(jīng)細(xì)胞凋亡過(guò)程的細(xì)胞類型傾向。每個(gè)芯片上有一組對(duì)照組，其數(shù)據(jù)分析可使用ΔΔCT相對(duì)量化的方法,評(píng)估逆轉(zhuǎn)錄性能和聚合酶鏈反應(yīng)性能的評(píng)估。使用SYBR?GREEN實(shí)時(shí)PCR和microrna qPCR芯片,可以很容易地、可靠地分析與細(xì)胞凋亡有關(guān)的miRNA。
miScript microrna的PCR芯片用于分子生物學(xué)的應(yīng)用程序。本產(chǎn)品不用于診斷,預(yù)防和治療的疾病。
Anti-Apoptotic:miR-106b-5p, miR-141-3p, miR-183-5p, miR-186-3p, miR-210, miR-214-3p, miR-338-3p, miR-378a-3p, miR-98-5p, miR-133a, miR-181a-5p, miR-21-5p, miR-25-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p.
Pro-Apoptotic:miR-128, miR-143-3p, miR-144-3p, miR-200c-3p, miR-205-5p, miR-206, miR-212-3p, miR-218-5p, miR-26a-5p, miR-26b-5p, miR-27a-3p, miR-30c-5p, miR-30e-5p, miR-31-5p, miR-365a-3p, miR-409-3p, miR-449a, miR-708-5p, let-7c, let-7g, miR-1, miR-101-3p, miR-133b, miR-149-3p, miR-153, miR-15b-5p, miR-16-5p, miR-181b-5p, miR-195-5p, miR-203a, miR-204-5p, miR-29a-3p, miR-29c-3p, miR-34a-5p, miR-497-5p, miR-512-5p.
Either Anti-Apoptotic or Pro-Apoptotic:let-7e-5p, miR-23a-3p, miR-24-3p, miR-34c-5p, let-7a-5p, miR-29b-3p.
Targets Anti-Apoptotic Genes:let-7c, let-7g, miR-1, miR-101-3p, miR-122-5p, miR-133a, miR-133b, miR-193b, miR-194-5p, miR-204-5p, miR-491-5p, miR-512-5p, miR-542-3p, miR-7-5p, miR-9-5p.
Targets Pro-Apoptotic Genes:miR-134, miR-221-3p, miR-222-3p, miR-32-5p.
Targets Both Anti-Apoptotic & Pro-Apoptotic Genes:let-7a-5p, miR-125a-5p, miR-125b-5p, miR-1285-3p, miR-145-5p, miR-146a-5p, miR-149-3p, miR-153, miR-15a-5p, miR-15b-5p, miR-16-5p, miR-17-5p, miR-181a-5p, miR-181b-5p, miR-181c-5p, miR-181d, miR-185-5p, miR-192-5p, miR-193a-5p, miR-195-5p, miR-203a, miR-20a-5p, miR-21-5p, miR-25-3p, miR-29a-3p, miR-29b-3p, miR-29c-3p, miR-30a-5p, miR-30b-5p, miR-30d-5p, miR-34a-5p, miR-451a, miR-497-5p, miR-92a-3p

實(shí)驗(yàn)原理與流程

What miScript miRNA PCR Arrays offer?
   Guaranteed Performance* - ready-to-use for gene expression analysis
   Time & Cost Saving - less than 30 min hands-on time to analyze 84 or 372 miRNA
   Simple Data Analysis - easy-to-use Excel-Based Data Analysis Template 

細(xì)胞凋亡miRNA PCR芯片 Apoptosis miScript miRNA PCR Array,細(xì)胞凋亡miRNA PCR芯片 Apoptosis miScript miRNA PCR Array

細(xì)胞凋亡miRNA PCR芯片 Apoptosis miScript miRNA PCR Array信息由上海英拜生物科技有限公司為您提供，如您想了解更多關(guān)于細(xì)胞凋亡miRNA PCR芯片 Apoptosis miScript miRNA PCR Array報(bào)價(jià)、型號(hào)、參數(shù)等信息，歡迎來(lái)電或留言咨詢。

注：該產(chǎn)品未在中華人民共和國(guó)食品藥品監(jiān)督管理部門(mén)申請(qǐng)醫(yī)療器械注冊(cè)和備案，不可用于臨床診斷或治療等相關(guān)用途