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Recombinant Mouse FLRG Protein, CF 25 UG

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Recombinant Mouse FLRG Protein, CF 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細(xì)說明

    • Purity

      >90%, by SDS-PAGE under reducing conditions and visualized by silver stain

    • Endotoxin Level

      <1.0 EU per 1 μg of the protein by the LAL method.  

    • Activity

      Measured by its ability to neutralize Activin-mediated erythroid differentiation of K562 human chronic myelogenous leukemia cells. The ED    50 for this effect is 3-15 ng/mL in the presence of 7.5 ng/mL rhActivin A.

    • Source

      Mouse myeloma cell line, NS0-derived Val24-Val256, with a C-terminal 6-His tag

    • Accession #

    • N-terminal Sequence    
      Analysis

      Val24

    • Predicted Molecular Mass

      26 kDa

    • SDS-PAGE

      38 kDa, reducing conditions

    Carrier Free

    What does CF mean?

    CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

    What formulation is right for me?

    In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

    1255-F3/CF

     

    1255-F3

    Formulation Lyophilized from a 0.2 μm filtered solution in PBS.


    Formulation Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

    Reconstitution Reconstitute at 100 μg/mL in sterile PBS.


    Reconstitution Reconstitute at 10 μg/mL in sterile PBS containing at least 0.1% human or bovine serum albumin.

    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.


    Shipping The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    Stability & Storage:       Use a manual defrost freezer and avoid repeated freeze-thaw cycles.      

    • 12 months from date of receipt, -20 to -70 °C as supplied.

    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.


    Stability & Storage:       Use a manual defrost freezer and avoid repeated freeze-thaw cycles.      

    • 12 months from date of receipt, -20 to -70 °C as supplied.

    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

    • 3 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Follistatin-related Gene Protein/FLRG

    Follistatin-Related Gene Protein (FLRG), also known as follistatin-like 3 (FSTL3) is a glycoprotein belonging to the follistatin-module protein family. Mouse FLRG cDNA encodes a 256 amino acid (aa) residue protein with a putative 23 aa signal peptide, an N-terminal domain, two cysteine-rich follistatin-like domains (FS) and a C-terminal acidic domain. Compared to follistatin, FLRG lacks the third FS domain found in follistatin. In addition, FLRG also lacks the heparin-binding domain found within the first amino-terminal FS domain of follistatin. Mouse and human FLRG share approximately 83% aa sequence homology. Like follistatin, FLRG has been shown to bind and inhibit the activities of TGF-beta family ligands including activin, BMP-2, -6, -7 and GDF-8/myostatin. While both FLRG and follistatin are located in a wide and overlapping range of adult and fetal tissue, their sites of peak expression differ: FLRG most highly in heart, lung, kidney, placenta and testis, while follistatin is highest in ovary and pituitary. The expression of FLRG is upregulated by TGF-beta and activin signaling through Smad proteins. Although FLRG is a secreted protein in many cell types, it has also been localized to the nuclear compartment in HeLa, 293 and CHO cells (1 - 5).

    • References:

      1. Tsuchida, K. et al. (2000) J. Biol. Chem. 275:40778.

      2. Sidis, Y. et al. (2002) Endocrinology 143:1613.

      3. Tortoriello, D.V. et al. (2001) Endocrinology 142:3426.

      4. Hill, J. et al. (2002) J. Biol. Chem. 277:40735.

      5. Bartholin, L. et al. (2001) Oncogene 20:5409.

    • Entrez Gene IDs:

      10272 (Human); 83554 (Mouse)

    • Alternate Names:

      FLRG; FLRGFSRP; follistatin-like 3 (secreted glycoprotein); Follistatin-like 3; Follistatin-like protein 3; Follistatin-related gene protein; follistatin-related protein 3; FSTL3





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