• Purity

  >90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

• Endotoxin Level

  <1.0 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to enhance BMP-2 induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. Nobta, M.     et al. (2005) J. Biol. Chem.     280:15842. The ED    ₅₀ for this effect is 0.5-2.0 μg/mL.

• Source

  Chinese Hamster Ovary cell line, CHO-derived

  Human Jagged 1 (Ser32-Ser1046) Accession # P78504	IEGRMD	Human IgG1 (Pro100-Lys330)
  N-terminus		C-terminus

• Accession #

• N-terminal Sequence    
  Analysis

  Ser32

• Structure / Form

  Disulfide-linked homodimer

• Predicted Molecular Mass

  137 kDa

• SDS-PAGE

  170-180 kDa, under reducing conditions

Background: Jagged 1

Jagged 1 is a 180 kDa type I transmembrane glycoprotein and member of the Delta-Serrate-Lag-2 (DSL) family of ligands that activate LIN12/Notch proteins. Human Jagged 1 is synthesized as a 1218 amino acid (aa) precursor that contains a 33 aa signal sequence, a 1034 aa extracellular domain (ECD), a 26 aa transmembrane segment, and a 125 aa cytoplasmic region (SwissProt # P78504). The ECD contains a DSL domain (aa 185-229), a cysteine-rich region, 15 EGF-like repeats, of which many bind calcium, and nine potential sites for N-linked glycosylation. Mature human Jagged 1 is 97% and 96% aa identical to mature mouse and rat Jagged 1, respectively. Jagged 1 is widely expressed in adult and fetal tissues. Jagged-Notch signaling specifies cell fate, regulates pattern formation, defines boundaries between different cell types, and modulates cell proliferation and differentiation, especially during hematopoiesis, myogenesis, neurogenesis, and development of vasculature (1-8). Mutations in human Jagged 1 are the cause of Alagille syndrome, an autosomal-dominant disorder characterized by intrahepatic cholestasis and abnormalities of heart, eye, vertebrae, as well as characteristic facial appearance (9-10).

• References:

1. Sainson, R.C. and A.L. Harris (2008) Angiogenesis 11:41.

2. Cordle, J. et al. (2008) Nat. Struct. Mol. Biol. 15:849.

3. Artavamis-Tsokanas, S. et al. (1999) Science 284:770.

4. Lai, E.C. (2004) Development 131:965.

5. Milner, L.A. et al. (1994) Blood 83:2057.  

6. Nyfeler, Y. et al. (2005) EMBO J. 24:3504

7. Linheng, L.         et al. (1998) Immunity         8:43.

8. Iso, T.         et al. (2003) Arterioscler. Thromb. Vasc. Biol.         23:543.

9. Oda, T.         et al. (1997) Nat. Genet.         16:235.

10. Oda, T.         et al. (1997) Genomics         43:376.

• Entrez Gene IDs:

  182 (Human); 29146 (Rat)

• Alternate Names:

  AGS; AHDMGC104644; Alagille syndrome; AWS; CD339 antigen; CD339; HJ1; JAG1; Jagged 1; Jagged1; JAGL1; protein jagged-1