• Purity

  >95%, by SDS-PAGE under reducing conditions and visualized by silver stain

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by the ability of the immobilized protein to support the adhesion of BCE C/D?1b bovine corneal endothelial cells. The ED    ₅₀ for this effect is 0.06-0.24 ug/mL.

• Source

  Human embryonic kidney cell, HEK293-derived Leu18-Ser705, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Leu18

• Predicted Molecular Mass

  75.1 kDa

• SDS-PAGE

  80-105 kDa, reducing conditions

Background: Protocadherin-17

Human Protocadherin 17 (PCDH17; also called Protocadherin?68) is an approximately 1159 amino acid (aa) glycoprotein belonging to the δ2 subgroup of protocadherins, type I transmembrane proteins that exhibit calcium?dependent but relatively weak homophilic adhesion (1?3). Like other δ2 protocadherins, PCDH17 cDNA encodes a signal sequence (aa 1?17), six cadherin domains of about 100 aa each (aa 18?695), a transmembrane sequence and a cytoplasmic domain (1). It contains an extracellular RGD cell attachment motif that may mediate integrin adhesion (3). Typical of protocadherins, an alternately spliced 889 aa form of human PCDH17 diverges at aa 876 within the cytoplasmic domain and lacks two conserved cytoplasmic motifs (1, 2). Within the extracellular domain, human PCDH17 shares 98% aa sequence identity with mouse and bovine, 99% with rat and equine, and 97% with porcine PCDH17. PCDH17 is constitutively expressed in the central nervous system (3). It is one of several PCDH molecules that shows region specificity in the basal ganglia, forming gradients (3). Early in postnatal life, it is preferentially expressed in motor and auditory cortices and is correlated with thalamo?cortical and intrahippocampal circuits (3?5). Its expression is increased in the Broadman area of the brain in subjects with schizophrenia (3). PCDH17 is frequently silenced in squamous cell carcinomas such as those in the esophagus and larynx, especially if they are poorly differentiated (3, 6, 7). Ectopic PCDH10 expression reduces tumor cell proliferation and migration (6).

• References:

1. Redies, C. et al. (2005) Cell. Mol. Life Sci. 62:2840.

2. Vanhalst, K. et al. (2005) Cell. Mol. Life Sci. 62:1247.

3. Kim, S. Y. et al. (2011) Cell Adh. Migr. 5:97.

4. Kim, S. Y. et al. (2007) Neuroscience 147:996.

5. Kim, S. Y. et al. (2010) Neuroscience 170:189.

6. Haruki, S. et al. (2010) Carcinogenesis 31:1027.

7. Giefing, M. et al. (2011) Genes Chromosomes Cancer 50:154.

• Entrez Gene IDs:

  27253 (Human); 219228 (Mouse); 306055 (Rat)

• Alternate Names:

  PCDH17; PCDH68; PCDH68protocadherin-68; PCH68; PCH68Protocadherin-68; protocadherin 17; protocadherin 68; Protocadherin17; Protocadherin-17