• Purity

  >85%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie? Blue Staining.

• Endotoxin Level

  <0.10 EU per 1 μg of the protein by the LAL method.  

• Activity

  Measured by its binding ability in a functional ELISA. Immobilized rhAnnexin-A6 can bind rhDecorin with an apparent K    _D <2 nM.  

• Source

  E. coli-derived Ala2-Asp673, with a C-terminal 6-His tag

• Accession #

• N-terminal Sequence    
  Analysis

  Ala2

• Predicted Molecular Mass

  76.5 kDa

• SDS-PAGE

  70 kDa, reducing conditions

Background: Annexin A6

The Annexins comprise a family of proteins that are involved in many aspects of cellular membrane dynamics and the regulation of membrane-associated proteins. They are characterized by multiple repeats of 60 - 70 amino acids (aa) which assemble into a curved disc-like domain that binds to membrane phospholipids in a calcium-dependent manner (1). Human Annexin A6, also known as Annexin VI, Lipocortin VI, and Chromobindin 20, is a 68 kDa protein that consists of eight tandem annexin repeats (2). Alternate splicing results in the deletion of 6 aa at the start of the seventh repeat (3). Human Annexin A6 shares 95% aa sequence identity with mouse and rat Annexin A6. Within the cell, Annexin A6 is involved in the regulation of membrane vesicle trafficking and chloride and calcium ion conductance (1, 4). Annexin A6 is also secreted into the bile and is present on the cell surface (5 - 7). Annexin A6 binds to chondroitin sulfate in a calcium-dependent manner and to heparan sulfate independently of calcium (6, 8). These interactions enable cell surface Annexin A6 to mediate adhesion such as that between macrophages and hepatic sinusoidal endothelial cells (6, 7).

• References:

1. Gerke, V. et al. (2005) Nat. Rev. Mol. Cell Biol. 6:449.

2. Crompton, M.R. et al. (1988) EMBO J. 7:21.

3. Smith, P.D. et al. (1994) Proc. Natl. Acad. Sci. 91:2713.

4. Grewal, T. et al. (2006) Bioessays 28:1211.

5. Thorin, B. et al. (1995) Biochem. Biophys. Res. Commun. 209:1039.

6. Takagi, H. et al. (2002) J. Cell Sci. 115:3309.

7. Tressler, R.J. et al. (1994) Exp. Cell Res. 215:395.

8. Ishitsuka, R. et al. (1998) J. Biol. Chem. 273:9935.

• Entrez Gene IDs:

  309 (Human); 11749 (Mouse); 79125 (Rat)

• Alternate Names:

  67 kDa calelectrin; Annexin A6; annexin VI (p68); Annexin VI; Annexin-6; ANX6; ANXA6; calcium-binding protein p68; Calelectrin; calphobindin II; calphobindin-II; CBP68; chromobindin-20; CPB-II ; CPB-II; Lipocortin VI; p68; p70; Protein III