• Species Reactivity

  Mouse

• Specificity

  Detects mouse Rae?1 epsilon in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant mouse Rae-1 alpha, beta, gamma, or δ is observed. By flow cytometry, no cross-reactivity with mouse Rae-1 alpha or mouse Rae-1 gamma.

• Source

  Monoclonal Rat IgG2a Clone # 205001

• Immunogen

  BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 350

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  BaF3 mouse pro-B cell line transfected with mouse Rae-1 epsilon

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Rae-1 epsilon

Rae-1 epsilon is a member of a family of cell-surface proteins that function as ligands for mouse NKG2D. Other family members are designated Rae-1 alpha, beta, gamma, and δ. Amino acid sequence identity within this family ranges from 88?95%. The Rae-1 proteins are distantly related to MHC class I proteins, but they possess only the alpha 1 and alpha 2 Ig?like domains, and they have no capacity to bind peptide or interact with beta 2-microglobulin. The genes encoding these proteins are not found within the Major Histocompatibility Complex on mouse chromosome 17, but rather map to mouse chromosome 10. The Rae-1 proteins are anchored to the membrane via a GPI?linkage. The name of this family derives from the original identification of these proteins as the product of retinoic acid early inducible transcripts. Rae-1 expression is developmentally controlled. Transcripts were observed in the brain/head region of day 10?14 embryos but disappeared by day 18. Rae-1 transcripts were detected in several transformed cell lines but are absent from most normal adult tissues. All Rae-1 family members bind to mouse NKG2D, an activating receptor expressed on NK cells and some T cell subsets, resulting in the activation of cytolytic activity and/or cytokine production by these effector cells. Ectopic expression of Rae-1 on mouse tumor cell lines resulted in the in vivo rejection of the tumors (1?7).

• References:

1. Zou, Z. et al. (1996) J. Biochem (Tokyo) 119:319.

2. Diefenbach, A. et al. (2000) Nature Immunol. 1:119.

3. Cerwenka, A. et al. (2000) Immunity 12:721.

4. Cerwenka, A. et al. (2001) Proc. Natl. Acad. Sci. USA 98:11521.

5. Diefenbach, A. et al. (2001) Nature 413:165.

6. Champsaur, M. et al. (2010) J. Immunol. 185:157.

7. Markiewicz M. et al. (2012) Immunity 36:132.

• Long Name:

  Retinoic Acid Early Transcript 1 epsilon

• Entrez Gene IDs:

  8480 (Human); 379043 (Mouse)

• Alternate Names:

  Rae1 epsilon; Rae-1 epsilon