• Species Reactivity

  Human, Mouse

• Specificity

  Detects mouse MMP?24/MT5-MMP in direct ELISAs and detects human and mouse MMP?24/MT5-MMP in Western blots. In Western blots, less than 5% cross-reactivity with recombinant mouse (rm) MMP-3, rmMMP-9, and the catalytic domains of recombinant human MMP?14, 15, and 16 is observed.

• Source

  Monoclonal Rat IgG    _2A Clone # 143908

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant mouse MMP?24/MT5-MMP    
  Arg125-Gln296    
  Accession # Q9R0S2

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  A172 human glioblastoma cell line and bEnd.3 mouse endothelioma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: MMP-24/MT5-MMP

MMP-24, also known as MT5-MMP, is a 62-65 kDa member of the M10 metallopeptidase family, a subfamily of MMPs. It is a type I transmembrane glycoprotein that is converted into a 58 kDa active form (amino acids [aa] 129-618) following removal of its propeptide. Proteolytic cleavage after Arg554 generates a 52 kDa active, soluble form that undergoes further cleavage in to 27-34 kDa fragment. MMP-24 has restricted expression, being found on neurons, mast cells, neural stem cells and ependymal cells lining the ventricles of the brain. It is known to cleave chondroitin sulfate, dermatan sulfate, pro-MMP-2, and N-Cadherin. Its activation of MMP-2 likely contributes to neurite outgrowth, while its cleavage of N-Cadherin in-trans between neural stem cells and ependymal initiates stem cell expansion and proliferation. Over amino acids 125-296, mouse MMP-24 shares 100% and 99% aa sequence identity with rat and human MMP-24, respectively.

• Long Name:

  Matrix Metalloproteinase 24/Membrane Type 5 MMP

• Entrez Gene IDs:

  10893 (Human); 17391 (Mouse)

• Alternate Names:

  EC 3.4.24; EC 3.4.24.-; EC 3.4.24.80; matrix metallopeptidase 24 (membrane-inserted); matrix metalloproteinase 24 (membrane-inserted); matrix metalloproteinase-24; membrane-type 5 matrix metalloproteinase; Membrane-type matrix metalloproteinase 5; Membrane-type-5 matrix metalloproteinase; MMP24; MMP-24; MT5MMP; MT5-MMP; MT5-MMPMMP25; MT-MMP 5; MTMMP5; MT-MMP5