• Species Reactivity

  Human

• Specificity

  Detects human M-CSF R/CD115 in direct ELISAs and Western blots. Does not cross-react with recombinant human (rh) GM-CSF R alpha or    
  rhGM-CSF R beta.

• Source

  Monoclonal Mouse IgG1 Clone # 61708

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human M-CSF R/CD115    
  Ile20-Glu512 (Pro54Ala)    
  Accession # P07333.2

• Formulation

  Supplied 0.2 mg/mL in a saline solution containing BSA and Sodium Azide.

• Label

  Alexa Fluor 750

Applications

• 
  

  Recommended    
  Concentration

  Sample

• Flow Cytometry

  0.25-1 μg/10    ⁶ cells

  Human peripheral blood monocytes

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: M-CSF R/CD115

M-CSF receptor, the product of the c-fms proto-oncogene, is a member of the type III subfamily of receptor tyrosine kinases that also includes receptors for SCF and PDGF. These receptors each contain five immunoglobulin-like domains in their extracellular domain (ECD) and a split kinase domain in their intracellular region (1?4). M-CSF receptor is expressed primarily on cells of the monocyte/macrophage lineage, dendritic cells, stem cells and in the developing placenta (1). Human M-CSF receptor cDNA encodes a 972 amino acid (aa) type I membrane protein with a 19 aa signal peptide, a 493 aa extracellular region containing the ligand-binding domain, a 25 aa transmembrane domain, and a 435 aa cytoplasmic domain. The human M-CSF R ECD shares 60%, 64%, 72%, 75%, 75%, and 76% aa identity with mouse, rat, bovine, canine, feline, and equine M-CSF R, respectively. Activators of protein kinase C induce TACE/ADAM17 cleavage of the M-CSF receptor, releasing the functional ligand-binding extracellular domain (5). M-CSF binding induces receptor homodimerization, resulting in transphosphorylation of specific cytoplasmic tyrosine residues and signal transduction (6). The intracellular domain of activated M-CSF R binds more than 150 proteins that affect cell proliferation, survival, differentiation and cytoskeletal reorganization. Among these, PI3Kinase, P42/44 ERK, and c-Cbl are key transducers of M-CSF R signals (3, 4). M-CSF R engagement is continuously required for macrophage survival and regulates lineage decisions and maturation of monocytes, macrophages, osteoclasts and DC (3, 4). M-CSF R and integrin  alpha _v beta ₃ share signaling pathways during osteoclastogenesis and deletion of either causes osteopetrosis (7, 8). In the brain, microglia expressing increased M-CSF R are concentrated with Alzheimers a beta peptide, but their role in pathogenesis is unclear (9, 10).

• References:

1. deParseval, N. et al. (1993) Nucleic Acids Res. 21:750.

2. Rothwell, V.M. and L.R. Rohrschneider (1987) Oncogene Res. 1:311.

3. Chitu, V. and E.R. Stanley (2006) Curr. Opin. Immunol. 18:39.

4. Ross, F.P. and S.L. Teitelbaum (2005) Immunol. Rev. 208:88.

5. Rovida, E. et al. (2001) J. Immunol. 166:1583.

6. Yeung, Y. et al. (1998) J. Biol. Chem. 273:17128.

7. Dai, X. et al. (2002) Blood 99:111.

8. Faccio, R. et al. (2003) J. Clin. Invest. 111:749.

9. Li, M. et al. (2004) J. Neurochem. 91:623.

10. Mitrasinovic, O.M. et al. (2005) J. Neurosci. 25:4442.

• Long Name:

  Macrophage Colony Stimulating Factor Receptor

• Entrez Gene IDs:

  1436 (Human)

• Alternate Names:

  CD115 antigen; CD115; c-fms; colony stimulating factor 1 receptor; CSF1R; CSF-1-R; CSFR; EC 2.7.10.1; FMS proto-oncogene; FMSFIM2; macrophage colony stimulating factor I receptor; macrophage colony-stimulating factor 1 receptor; McDonough feline sarcoma viral (v-fms) oncogene homolog; M-CSF R; MCSFR; M-CSFR; Proto-oncogene c-Fms