• Species Reactivity

  Human

• Specificity

  Detects human Glucuronosyltransferase 1A1/UGT1A1 in ELISAs.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 856754

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Glucuronosyltransferase 1A1/UGT1A1    
  Leu60-Thr168    
  Accession # P22309

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  2 μg/mL

  See below

• 
  

• Simple Western

  20 μg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glucuronosyltransferase 1A1/UGT1A1

UGT1A1 (UDP-glucuronosyltransferase 1-A1; also UDPGT 1-1 and HUG-BR1) is a 52-57 kDa member of the UGT1A subfamily, UGT family of enzymes. UGT1A1 is expressed by the liver, and catalyzes the conjugation of glucuronic acid (GA) from UDPGA to lipophilic acceptors such as (anti-cancer) drugs and bilirubin. Addition of glucuronic acid increases target solubility and facilitates elimination. Mature human UGT1A1 is a 508 amino acid (aa) type I transmembrane ER glycoprotein. It contains a 465 aa luminal domain (aa 26-490) plus a 26 aa cytoplasmic region. The luminal domain is unusual in that aa 157-176 are embedded in the ER membrane. Amino acids 29-444 contain the enzyme active site. The cytoplasmic tail appears to mediate noncovalent homodimerization, and heterodimerization with UGT2B. The signal sequence (aa 1-25) is normally cleaved, but a Lys15Arg mutation blocks insertion into the ER membrane. There are multiple point mutations that impact enzyme activity. One potential splice form is reported that shows a six aa substitution for aa 289-533. Over aa 60-186, human UGT1A1 shares 64% aa identity with mouse UGT1A1.

• Long Name:

  UDP Glucuronosyltransferase 1A1

• Entrez Gene IDs:

  54658 (Human); 394436 (Mouse); 24861 (Rat)

• Alternate Names:

  bilirubin UDP-glucuronosyltransferase 1-1; bilirubin UDP-glucuronosyltransferase isozyme 1; Bilirubin-specific UDPGT isozyme 1; GNT1; GNT1EC 2.4.1.17; HUG-BR1; UDP glucuronosyltransferase 1 family, polypeptide A1; UDP glycosyltransferase 1 family, polypeptide A1; UDP-glucuronosyltransferase 1-1; UDP-glucuronosyltransferase 1-A; UDP-glucuronosyltransferase 1A1; UDPGT 1-1; UDPGT; UG-BR1; UGT1; UGT1*1; UGT1.1; UGT1-01; UGT1A; UGT-1A; UGT1A1; UGT1AUDPGT 1-1; UGT1BILIQTL1