• Species Reactivity

  Human, Mouse

• Specificity

  Recognizes a carbohydrate epitope of SSEA-4 (1, 2).

• Source

  Monoclonal Mouse IgG    ₃ Clone # MC-813-70

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  2120Ep human embryonal carcinoma cell line

• Formulation

  Lyophilized from a 0.2 μm filtered solution in Tris and NaCl with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  NTera?2 human testicular embryonic carcinoma cell line

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SSEA-4

SSEA-4 is expressed on the surface of human embryonal carcinoma (EC) cells (the pluripotent stem cells of teratocarcinomas), human embryonic germ cells (EG), and human embryonic stem cells (ES). Expression of SSEA-4 is down-regulated following differentiation of human EC cells. In contrast, the differentiation of murine EC and ES cells may be accompanied by an increase in SSEA-4 expression (1-4).

• References:

1. Shevinsky, L.H. et al. (1982) Cell 30:697.

2. Kannagi, R. et al. (1983) EMBO J. 2:2355.

3. Thomson, J.A. and J.S. Odorico (2000) Trends Biotechnol. 18:53.

4. Draper, J.S. et al. (2002) J. Anat. 200:249.

• Long Name:

  Stage-specific Embryonic Antigen-4

• Alternate Names:

  SSEA4; SSEA-4