詳細說明
Species Reactivity
Mouse
Specificity
Detects mouse Frizzled-3 in direct ELISAs and Western blots. In flow cytometry and immunohistochemistry, also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross?reactivity with Frizzled-1, -4, -7 or -8 is observed.
Source
Monoclonal Rat IgG 2A Clone # 169310
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3
Met1-Val157
Accession # Q61086Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin
Applications
Recommended
ConcentrationSample
Western Blot
1 μg/mL
Recombinant Mouse Frizzled?3
Flow Cytometry
2.5 μg/10 6 cells
HEK293 human embryonic kidney cell line
Immunohistochemistry
8-25 μg/mL
Immersion fixed frozen sections of mouse embryo (E13-15)
Please Note: Optimal dilutions should be determined by each laboratory for each application. are available in the Technical Information section on our website.
Preparation and Storage
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Frizzled-3
Frizzled-3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.
Entrez Gene IDs:
7976 (Human); 14365 (Mouse)
Alternate Names:
frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3