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Mouse Frizzled-3 Biotinylated MAb (Clone 169310) 100 UG

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Mouse Frizzled-3 Biotinylated MAb (Clone 169310) 100 UG信息二維碼

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產品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Mouse

    • Specificity

      Detects mouse Frizzled-3 in direct ELISAs and Western blots. In flow cytometry and immunohistochemistry, also detects mouse Frizzled-3 on differentiated embryoid bodies derived from the mouse embryonic cell line, D3, and on human HEK 293 cells. In direct ELISAs, no cross?reactivity with Frizzled-1, -4, -7 or -8 is observed.

    • Source

      Monoclonal Rat IgG    2A Clone # 169310

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant mouse Frizzled-3    
      Met1-Val157    
      Accession # Q61086

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

    • Label

      Biotin

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      1 μg/mL

      Recombinant Mouse Frizzled?3


    • Flow Cytometry

      2.5 μg/10    6 cells

      HEK293 human embryonic kidney cell line


    • Immunohistochemistry

      8-25 μg/mL

      Immersion fixed frozen sections of mouse embryo (E13-15)


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: Frizzled-3

    Frizzled-3 is a member of the Frizzled family of G-protein coupled receptors that have seven transmembrane domains and an extracellular CRD. Frizzled proteins function as receptors for Wnt proteins.

    • Entrez Gene IDs:

      7976 (Human); 14365 (Mouse)

    • Alternate Names:

      frizzled (Drosophila) homolog 3; frizzled homolog 3 (Drosophila); Frizzled3; Frizzled-3; Fz-3; FZD3; hFz3





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