• Species Reactivity

  Human

• Specificity

  Detects human Fc gamma RIIIA/B (CD16) in direct ELISAs. In direct ELISAs, approximately 10% cross-reactivity with recombinant human Fc gamma RIIA and recombinant mouse Fc gamma RIII is observed.

• Source

  Monoclonal Mouse IgG    _2A Clone # 245536

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Fc gamma RIIIA/B (CD16)    
  Thr20-Gln208    
  Accession # O75015

• Formulation

  Supplied in a saline solution containing BSA and Sodium Azide.

• Label

  Allophycocyanin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  10 μL/10    ⁶ cells

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Shipping

  The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Protect from light.    Do not freeze.    

• 12 months from date of receipt, 2 to 8 °C as supplied.

Background: Fc gamma RIII (CD16)

Fc gamma RIIIA and B are low affinity receptors for polyvalent immune-complexed IgG. Fc gamma RIIIA is a transmembrane activating receptor expressed by NK cells, T cells, monocytes, and macrophages. Fc gamma RIIIB is a non-activating GPI-linked decoy receptor expressed on neutrophils and eosinophils.

• Long Name:

  Fc gamma Receptor III

• Entrez Gene IDs:

  14131 (Mouse); 102140945 (Cynomolgus Monkey)

• Alternate Names:

  Fc gamma RIII; FcgRIII