• Species Reactivity

  Human, Mouse, Rat

• Specificity

  Vimentin antibodies are ideal for immunocytochemistry colocalization studies in intermediate filaments. The unconjugated antibody detects human Vimentin in Western blots.

• Source

  Monoclonal Rat IgG    _2A Clone # 280618

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Vimentin    
  Ser2-Glu466    
  Accession # P08670

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Vimentin

Vimentin is a 57 kDa class III intermediate filament (IF) protein that belongs to the intermediate filament family. It is the predominant IF in cells of mesenchymal origin such as vascular endothelium and blood cells (1-3). The human Vimentin cDNA encodes a 466 amino acid (aa) protein that contains head and tail regions with multiple regulatory Ser/Thr phosphorylation sites, and a central rod domain with three coiled-coil regions separated by linkers (1, 2). Human Vimentin shares 97-98% aa identity with mouse, rat, ovine, bovine, and canine Vimentin. Sixteen Vimentin coiled-coil dimers self-assemble to form intermediate (10-12 nm wide) filaments (4). These filaments then anneal longitudinally to form non-polarized fibers that support cell structure and withstand stress (4). IF fibers are highly dynamic, and half-life depends on the balance between kinase and phosphatase activity. For example, phosphorylation followed by dephosphorylation drives IF disintegration, followed by reorganization during mitosis (1, 5, 6). Interactions of head and tail domains link IFs with other structures such as actin and microtubule cytoskeletons (7). Vimentin is involved in positioning autophagosomes, lysosomes and the Golgi complex within the cell (8). It facilitates cell migration and motility by recycling internalized trailing edge integrins back to the cell surface at the leading edge (9-11). Vimentin helps maintain the lipid composition of cellular membranes, and caspase cleavage of Vimentin is a key event in apoptosis (8, 12). Phosphorylation promotes secretion of Vimentin by TNF-alpha -stimulated macrophages (13). Extracellular Vimentin has been shown to associate with several microbes, and appears to promote an antimicrobial oxidative burst (13, 14). Cell-associated Vimentin can also interact with NKp46 to recruit NK cells to tuberculosis-infected monocytes (15).

• References:

1. Omary, M.B. et al. (2006) Trends Biochem. Sci. 31:383.

2. Ivaska, J. et al. (2007) Exp. Cell Res. 313:2050.

3. Ferrari, S. et al. (1986) Mol. Cell. Biol. 6:3614.

4. Sokolova, A.V. et al. (2006) Proc. Natl. Acad. Sci. USA 103:16206.

5. Eriksson, J.E. et al. (2004) J. Cell Sci. 117:919.

6. Li, Q-F. et al. (2006) J. Biol. Chem. 281:34716.

7. Esue, O. et al. (2006) J. Biol. Chem. 281:30393.

8. Styers, M.L. et al. (2005) Traffic 6:359.

9. McInroy, L. and A. Maata (2007) Biochem. Biophys. Res. Commun. 360:109.

10. Nieminen, M. et al. (2006) Nat. Cell Biol. 8:156.

11. Ivaska, J. et al. (2005) EMBO J. 24:3834.

12. Byun, Y. et al. (2001) Cell Death Differ. 8:443.

13. Mor-Vaknin, N. et al. (2003) Nat. Cell Biol. 5:59.

14. Zou, Y. et al. (2006) Biochem. Biophys. Res. Commun. 351:625.

15. Garg, A. et al. (2006) J. Immunol. 177:6192.

• Entrez Gene IDs:

  7431 (Human); 22352 (Mouse); 81818 (Rat)

• Alternate Names:

  FLJ36605; VIM; Vimentin