• Species Reactivity

  Human

• Specificity

  Detects human E-Cadherin in direct ELISAs.

• Source

  Monoclonal Mouse IgG    _2B Clone # 180224

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human E-Cadherin    
  Asp155-Ile707    
  Accession # P12830

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

• Label

  Biotin

Applications

• Recommended    
  Concentration

  Sample

• Flow Cytometry

  2.5 μg/10    ⁶ cells

  MCF?7 human breast cancer cell line stained in buffer containing Ca    ²⁺ and Mg    ²⁺  

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: E-Cadherin

Epithelial (E)-Cadherin (ECAD), also known as cell-CAM120/80 in the human, uvomorulin in the mouse, Arc-1 in the dog, and L-CAM in the chicken, is a member of the Cadherin family of cell adhesion molecules. Cadherins are calcium?dependent transmembrane proteins which bind to one another in a homophilic manner. On their cytoplasmic side, they associate with the three catenins, alpha, beta, and gamma (plakoglobin). This association links the cadherin protein to the cytoskeleton. Without association with the catenins, the cadherins are non-adhesive. Cadherins play a role in development, specifically in tissue formation. They may also help to maintain tissue architecture in the adult. E?Cadherin may also play a role in tumor development, as loss of E?Cadherin has been associated with tumor invasiveness. E?Cadherin is a classical cadherin molecule. Classical cadherins consist of a large extracellular domain which contains DXD and DXNDN repeats responsible for mediating calcium?dependent adhesion, a single-pass transmembrane domain, and a short carboxy-terminal cytoplasmic domain responsible for interacting with the catenins. E?Cadherin contains five extracellular calcium-binding domains of approximately 110 amino acids each.

• References:

1. Bussemakers, M.J.G. et al. (1993) Mol. Biol. Reports 17:123.

2. Overduin, M. et al. (1995) Science 267:386.

3. Takeichi, M. (1991) Science 251:1451.

• Entrez Gene IDs:

  999 (Human); 12550 (Mouse); 83502 (Rat)

• Alternate Names:

  Arc-1; CAD1; cadherin 1, E-cadherin (epithelial); cadherin 1, type 1, E-cadherin (epithelial); Cadherin-1; calcium-dependent adhesion protein, epithelial; CAM 120/80; CD324 antigen; CD324; CDH1; CDHE; cell-CAM 120/80; Cell-CAM120/80; ECAD; ECadherin; E-Cadherin; Epithelial cadherin; LCAM; L-CAM; UVOE-Cadherin; Uvomorulin