詳細(xì)說(shuō)明
Assay Type
Magnetic bead-based multiplex assay for the Luminex ? MAGPIX ? platform
Format
96-well microplate and antibody-coated magnetic beads*
Analytes Detected
Please see table below.
Performance Validation
For standard curves, intra-assay and inter-assay precision, recovery, and linearity data for the different analyte-specific beads, please see product datasheets for corresponding individual Bead Sets (below).
* Antibody-coated beads supplied in compatible Bead Sets, sold separately (see below).
Product Features
Simultaneously detect multiple analytes
Magnetic format allows for easier wash steps
User selects specific analytes of interest
Requires small sample size
Undergoes similar validation testing as Quantikine? ELISA Kits
Precise and Specific
Base Kit Contents
Standard Cocktail(s)
Bead Diluent
Biotin Antibody Diluent
Calibrator Diluent(s)
Streptavidin-PE
Wash Buffer Concentrate
Mixing Bottles
Round-bottom Microplate
Foil Plate Sealers
Bead Set Contents
Antibody-coated magnetic beads
Biotinylated detection antibody
Information for Compatiable Analytes
For performance data for the different analyte-specific beads, please see product datasheets for the compatible individual Bead Sets.
| Analyte | Catalog Number | Sensitivity (pg/mL) | High Standard Value* (pg/mL) | Microparticle Region |
| EMMPRIN | 5.6 | 25,700 | 30 | |
| MMP-1 | 1.1 | 18,300 | 20 | |
| MMP-2 | 12.6 | 63,100 | 19 | |
| MMP-3 | 7.3 | 25,700 | 21 | |
| MMP-7 | 6.6 | 55,850 | 22 | |
| MMP-8 | 16.6 | 59,700 | 25 | |
| MMP-9 | 13.7 | 73,100 | 26 | |
| MMP-10 | 3.2 | 31,000 | 27 | |
| MMP-12 | 0.7 | 6,700 | 28 | |
| MMP-13 | 63.5 | 324,000 | 29 | |
*A standard curve must be generated each time an assay is run, utilizing values from the Standard Value Card included in the Base Kit. | ||||
Validated Samples and Recommended Starting Dilution
| Analyte | Cell Culture Supernate | Serum | Heparin Plasma | Platelet-poor Heparin Plasma | Saliva | Urine |
| EMMPRIN | 1:5 | 1:5* | 1:5* | 1:5* | 1:40 | 1:10 |
| MMP-1 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-2 | N/R | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-3 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-7 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-8 | 1:5 | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-9** | 1:5 | 1:50 | N/R | 1:50 | 1:40 | 1:10 |
| MMP-10 | 1:5 | 1:5 | 1:5 | 1:5 | 1:40 | 1:10 |
| MMP-12 | 1:5 | 1:50 | 1:50 | 1:50 | 1:40 | 1:10 |
| MMP-13 | 1:5 | 1:5 | N/R | N/R | 1:40 | 1:10 |
N/R=This sample type is not recommended for use in this assay *When assaying serum and plasma samples, EMMPRIN cannot be multiplexed with MMP-7, MMP-8, MMP-10, MMP-12, or MMP-13. **MMP-9 is present in platelet granules and released upon platelet activation. Therefore, to measure circulating levels of this factor, platelet-free plasma should be collected for measurement. | ||||||
More Information about Magnetic Bead-Based Multiple Kits for the Luminex Platform
Magnetic multiplex kits are designed for use with the CCD Imager. Alternatively, kits can be used with the or Bio-Rad? Bio-Plex?, dual laser, flow-based sorting and detection platforms.
Analyte-specific antibodies are pre-coated onto color-coded magnetic microparticles. Microparticles, standards and samples are pipetted into wells and the immobilized antibodies bind the analytes of interest. After washing away any unbound substances, a biotinylated antibody cocktail specific to the analytes of interest is added to each well. Following a wash to remove any unbound biotinylated antibody, streptavidin-phycoerythrin conjugate (Streptavidin-PE), which binds to the biotinylated antibody, is added to each well. A final wash removes unbound Streptavidin-PE, the microparticles are resuspended in buffer and read using the Luminex MAGPIX Analyzer. A magnet in the analyzer captures and holds the superparamagnetic microparticles in a monolayer. Two spectrally distinct Light Emitting Diodes (LEDs) illuminate the beads. One LED identifies the analyte that is being detected and the second LED determines the magnitude of the PE-derived signal, which is in direct proportion to the amount of analyte bound. Each well is imaged with a CCD camera. Kits can also be used with Luminex 100/200 or a Bio-Rad Bio-Plex dual laser, flow-based systems.
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