• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse ZBTB7A/Pokemon in Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 466407

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human ZBTB7A/Pokemon    
  Met349-Val476    
  Accession # O95365

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ZBTB7A/Pokemon

ZBTB7A (zinc finger and BTB domain-containing 7A), also known as Pokemon, FBI-1 in human, LRF in mouse, and OCZF in rat, is a widely expressed nuclear protein that is overexpressed in many human cancers. It is a transcriptional repressor of ARF, c-fos, c-myc, Rb and others, including many extracellular matrix genes. These targets play important roles, both in normal differentiation and oncogenesis. Human ZBTB7A/Pokemon is a 584 amino acid (aa) protein with a BTB-POZ domain (aa 24?131) and four zinc finger repeats (aa 382?484). Human ZBTB7A/Pokemon shares 98% aa identity with mouse and rat within aa 349?476.

• Long Name:

  Zinc Finger and BTB Domain-Containing Protein 7A

• Entrez Gene IDs:

  51341 (Human); 16969 (Mouse); 117107 (Rat)

• Alternate Names:

  DKFZp547O146; Factor binding IST protein 1; Factor that binds to inducer of short transcripts protein 1; FBI-1; FBI-1POK erythroid myeloid ontogenic factor; FBI1TTF-I-interacting peptide 21; HIV-1 inducer of short transcripts binding protein; Leukemia/lymphoma-related factor; LRF; LRFHIV-1 1st-binding protein 1; lymphoma related factor; MGC99631; Pokemon; TIP21; ZBTB7A; ZBTB7zinc finger and BTB domain containing 7; zinc finger and BTB domain containing 7A; zinc finger and BTB domain containing 7A, HIV-1 inducer of short transcriptsbinding protein; zinc finger and BTB domain-containing protein 7A; Zinc finger protein 857A; ZNF857APOZ and Krueppel erythroid myeloid ontogenic factor