• Species Reactivity

  Human

• Specificity

  Detects human Chitobiase/CTBS in direct ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    ₁ Clone # 486725

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Chitobiase/CTBS    
  Thr39-Arg385    
  Accession # Q01459

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human Chitobiase/CTBS

• 
  

• Immunoprecipitation

  25 μg/mL

  Conditioned cell culture medium spiked with Recombinant Human Chitobiase/CTBS,

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Chitobiase/CTBS

Di-N-acetyl-chitobiase (CTBS) is a lysosomal glycosidase that is involved in the degradation of asparagine-linked glycoproteins. The amino acid sequence of human CTBS shares 99%, 86%, 82%, and 77% identity with that of chimpanzee, mouse, rat, and canine CTBS, respectively.

• Entrez Gene IDs:

  1486 (Human); 74245 (Mouse); 81652 (Rat)

• Alternate Names:

  Chitobiase; chitobiase, di-N-acetyl-; CTBdi-N-acetylchitobiase; CTBS; EC 3.2.1.-