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Human IgG Fc Alexa Fluor 405 MAb (Clone 97924) 25 TESTS

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Human IgG Fc Alexa Fluor 405 MAb (Clone 97924) 25 TESTS信息二維碼

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產品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects chimeric proteins containing a human IgG Fc region in direct ELISAs.

    • Source

      Monoclonal Mouse IgG    1 Clone # 97924

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      Mouse myeloma cell line NS0-derived recombinant human IgG    1 Fc region

    • Formulation

      Supplied in a saline solution containing BSA and Sodium Azide.

    • Label

      Alexa Fluor 405

    Applications

    • Recommended    
      Concentration

      Sample

    • Flow Cytometry

      5 μL/10    6 cells

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Flow Cytometry      
         

    Detection of IgG1 in HEK293 Human Cell Line Transfected with Human CD94 Fc Chimera by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with either human CD94 Fc Chimera (filled histogram) or irrelevant transfectants (open histogram) was stained with Mouse Anti-Human IgG Fc Alexa Fluor? 405?conjugated Monoclonal Antibody (Catalog # FAB110V). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # ) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (Catalog # ). View our protocol for .

    Preparation and Storage

    • Shipping

      The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

    • Stability & Storage

      Protect from light.    Do not freeze.    

      • 12 months from date of receipt, 2 to 8 °C as supplied.

    Background: IgG

    R&D Systems offers a range of secondary antibodies and controls for flow cytometry, immunohistochemistry, and Western blotting. We provide species-specific secondary antibodies that are available with a variety of conjugated labels.

    Our NorthernLights fluorescent secondary antibodies are bright and resistant to photobleaching. We are currently offering secondary antibodies recognizing mouse, rat, goat, sheep, and rabbit IgG as well as chicken IgY. These reagents are available with three distinct excitation and emission maxima, making them ideal for multi-color fluorescence microscopy.


    • Long Name:

      Immunoglobulin G

    • Alternate Names:

      IgG; immunoglobulin heavy constant gamma 1 (G1m marker)

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