• Species Reactivity

  Human

• Specificity

  Detects human Endothelin?1 C-Terminus. Recognizes the C-terminal region of mature Endothelin-1, -2, and -3 (amino acid residues 15?21).

• Source

  Monoclonal Rat IgG    _2B Clone # 3G10

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Human Endothelin-1

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• ELISA

  This clone has been shown to effectively function as an ELISA capture antibody.

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Endothelin-1

Endothelin-1, -2, and -3 are encoded by three separate genes as prepropolypeptides that are cleaved to yield inactive big endothelins. Big Endothelins are further cleaved to generate the 21 amino acid bioactive mature peptides. Endothelin-1 has potent vasoconstricting and angiogenic activity that is mediated through the 7TM receptors, ET-A and ET-B. Endothelin-1, -2, and -3 share identical amino acid sequences at their C-termini (residues 15?21).

• Entrez Gene IDs:

  1906 (Human); 13614 (Mouse); 24323 (Rat)

• Alternate Names:

  EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1