• Species Reactivity

  Mouse

• Specificity

  Detects mouse NgR in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross-react with recombinant human NgR.

• Source

  Monoclonal Rat IgG    _2B Clone # 202604

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant mouse NgR    
  Cys27-Ser447    
  Accession # Q99PI8

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Mouse Nogo Receptor/NgR Fc Chimera (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Nogo Receptor/NgR

NgR,also named reticulon 4 receptor, is a glycosylphosphoinositol (GPI)-anchored proteinthat belongs to the family of leucine-rich repeat proteins. NgR plays an essential role inmediating axon growth inhibition induced by the myelin-derived proteins Nogo, myelinassociatedglycoprotein (MAG), and myelin oligodendrocyte glycoprotein (Omgp). Uponligand binding, NgR associates with and activates the p75 neurotrophin receptor(p75NTR), a tumor necrosis factor superfamily member (TNFRSF16).

• Long Name:

  Nogo-66 Receptor/Reticulon 4 Receptor

• Entrez Gene IDs:

  65078 (Human); 65079 (Mouse)

• Alternate Names:

  NgR; NgR1; Nogo R; Nogo receptor; Nogo-66 receptor; NogoR; NOGORUNQ330/PRO526; reticulon 4 receptor; reticulon-4 receptor; RTN4R