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Human TBX2 MAb (Clone 393725) 25 UG

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Human TBX2 MAb (Clone 393725) 25 UG信息二維碼

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產(chǎn)品介紹

    基本參數(shù)

    詳細說明

    • Species Reactivity

      Human

    • Specificity

      Detects endogenous human TBX2 in Western blots at approximately 75 kDa.

    • Source

      Monoclonal Rat IgG    2B Clone # 393725

    • Purification

      Protein A or G purified from hybridoma culture supernatant

    • Immunogen

      E. coli-derived recombinant human TBX2    
      Thr593-Arg702    
      Accession # Q13207

    • Formulation

      Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

    • Label

      Unconjugated

    Applications

    • Recommended    
      Concentration

      Sample

    • Western Blot

      0.1 μg/mL

      See below


    Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

    Data Examples

    Western Blot      
         

    Detection of Human TBX2 by Western Blot. Western blot shows lysates of HEK293T human embryonic kidney cell line, JEG?3 human epithelial choriocarcinoma cell line, NTera?2 human testicular embryonic carcinoma cell line, and BG0IV human embryonic stem cells. PVDF membrane was probed with 0.1 μg/mL of Human TBX2 Monoclonal Antibody (Catalog # MAB5040) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # ). A specific band was detected for TBX2 at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using .

    Preparation and Storage

    • Reconstitution

      Reconstitute at 0.5 mg/mL in sterile PBS.

    • Shipping

      The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

    • Stability & Storage

      Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

      • 12 months from date of receipt, -20 to -70 °C as supplied.

      • 1 month, 2 to 8 °C under sterile conditions after reconstitution.

      • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

    Background: TBX2

    TBX2 (T-box transcription factor 2) is a member of a family of transcription factors that share the T-box DNA binding domain. TBX2, as well as other TBX proteins, is involved in remodeling of the heart myocardium through a complex spatial and temporal regulation. TBX2 is associated with tumorigenesis and anti-senescence properties by affecting several cyclin-dependent kinase inhibitors. The full-length protein contains 702 amino acids with a DNA binding domain from aa 99 to aa 277.

    • Long Name:

      T-box Transcription Factor 2

    • Entrez Gene IDs:

      6909 (Human); 21385 (Mouse); 303398 (Rat)

    • Alternate Names:

      FLJ10169; T-box 2; T-box protein 2; T-box transcription factor TBX2; TBX2




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