• Species Reactivity

  Human

• Specificity

  Detects human Lipocalin-1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, this antibody does not cross?react with recombinant human Lipocalin-2.

• Source

  Monoclonal Mouse IgG    _2A Clone # 223908

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  Mouse myeloma cell line NS0-derived recombinant human Lipocalin-1    
  His19-Asp176    
  Accession # P31025

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  Recombinant Human Lipocalin?1 (Catalog # )

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Lipocalin-1

Lipocalin-1, also known as tear prealbumin or von Ebner’s gland protein (VEGP), is encoded by the LCN1 gene (1 - 3). It is a member of the Lipocalin superfamily that binds many different classes of lipophylic chemicals (4). Lipocalin-1 contains three sequence motifs similar to the cystatins, a superfamily of cysteine protease inhibitors (5). In fact, it has been suggested that Lipocalin-1 is a physiological inhibitor of cysteine proteases and plays a role in the control of inflammatory processes in oral and ocular tissues (5). rhLipocalin-1 corresponds to the mature and secreted protein. It is a weak inhibitor of cysteine proteases such as cathepsin V, which is similar to rhCystatin S (R&D Systems, Catalog # 1296-PI).

• References:

1. Redl, B. et al. (1992) J. Biol. Chem. 267:20282.

2. Blaker, M. et al. (1993) Biochim. Biophys. Acta 1172:131.

3. Lassagne, H. and A.M. Gachon (1993) Exp. Eye Res. 56:605.

4. Redl, B. et al. (2000) Biochim. Biophys. Acta 1482:241.

5. van’t Hof, W. et al. (1997) J. Biol. Chem. 272:1837.

• Entrez Gene IDs:

  3933 (Human)

• Alternate Names:

  LCN1; lipocalin 1 (tear prealbumin); lipocalin 1-like 2; Lipocalin1; Lipocalin-1; MGC71975; PMFA; Tear lipocalin; Tear Prealbumin; TLC; TPVon Ebner gland protein; VEG protein; VEGP; VEGPlipocalin 1 (protein migrating faster than albumin, tear prealbumin)