• Species Reactivity

  Human

• Specificity

  Detects human TBX18 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human (rh) TBX2, 3, 5, 6, 20, or rhT-bet is observed.

• Source

  Monoclonal Mouse IgG    _2B Clone # 635305

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human TBX18    
  Ala384-Ser504    
  Accession # O95935

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX18

TBX18 is a 607 amino acid, 65 kDa (predicted) protein that is a member of the T-box family of developmentally expressed transcription factors. It is developmentally expressed in the anterior portion of the head, somites and limb buds. It is expressed on pluripotent progenitors in the developing epicardium where it is required for formation of the cardiac AV node, and can also contribute to fibroblast and smooth muscle formation. Within the region used as an immunogen, human TBX18 shares 93% amino acid identity with mouse and rat TBX18.

• Long Name:

  T-box Transcription Factor 18

• Entrez Gene IDs:

  9096 (Human); 76365 (Mouse); 315870 (Rat)

• Alternate Names:

  T-box 18; T-box protein 18; T-box transcription factor TBX18; TBX18