• Species Reactivity

  Human, Mouse

• Specificity

  Detects human and mouse SATB1 in ELISAs and Western blots.

• Source

  Monoclonal Mouse IgG    _2B Clone # 815427

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human SATB1    
  Met1-Ser260    
  Accession # Q01826

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Simple Western

  10 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.5 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: SATB1

SATB1 (special AT-rich sequence binding protein-1) is an approximately 103 kDa nuclear matrix-associated homeobox transcription factor that binds AT rich DNA sequences at scaffolding protein attachment and viral genome integration sites. It is a key factor integrating higher-order chromatin architecture with gene regulation. Specifically, it is involved in MHC class-I gene organization and chromatin organization in undifferentiated thymocytes. SATB1 is cleaved into 65-70 kDa and 25?30 kDa fragments during apoptosis. Human SATB1 shares 99% amino acid sequence identity with mouse and rat SATB1.

• Long Name:

  SATB Homeobox 1

• Entrez Gene IDs:

  6304 (Human); 20230 (Mouse); 316164 (Rat)

• Alternate Names:

  DNA-binding protein SATB1; SATB homeobox 1; SATB1; special AT-rich sequence binding protein 1 (binds to nuclearmatrix/scaffold-associating DNA's); Special AT-rich sequence-binding protein 1