• Species Reactivity

  Human

• Specificity

  Detects human Glutathione S-Transferase pi 1/GSTP1 in direct ELISAs and Western blots. Detects mouse and rat Glutathione S-Transferase pi 1/GSTP1 in Western Blots.    
   

• Source

  Monoclonal Mouse IgG    _2B Clone # 800027

• Purification

  Protein A or G purified from hybridoma culture supernatant

• Immunogen

  E. coli-derived recombinant human Glutathione S-Transferase pi 1/GSTP1    
  Met1-Glu210    
  Accession # AAC51280

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  0.25 μg/mL

  See below

• 
  

• Simple Western

  12.5 μg/mL

  See below

• 
  

• Immunocytochemistry

  8-25 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Sterile PBS to a final concentration of 0.5 mg/mL.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Glutathione S-Transferase pi 1/GSTP1

Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTP1 is a cytosolic protein that belongs to pi class of the GST superfamily. It is crystallized as a homodimer (1), but also exists in solution as an equilibrium mixture of monomer and dimer, depending on the protein concentration (2). Four genetic variants of GSTP1 with different enzymatic activities have been identified, which indicates the particular allelic form expressed in tissues could contribute to variation in catalytic efficiency and biological functions (3, 4). Human GSTP1 is present at elevated levels in many tumor cells, and has unique properties as a cancer marker (5). Genetic polymorphisms and expression patterns of GSTP1 have been associated with a variety of effects on human cancer, anti-cancer drug resistance, and asthma (6). In addition to its role as a drug-metabolizing enzyme, GSTP1 has ligand binding properties and regulates kinase signaling pathways through protein-protein interactions (7).

• References:

1. Reinemer, P. et al. (1992) J. Mol. Biol. 227:214.

2. Huang, Y.C. et al. (2008) J. Biol. Chem. 283:32880.

3. Ali-Osman, F. et al. (1997) J. Biol. Chem. 272:10004.

4. Hu, X. et al. (1998) Cancer Res. 58:5340.

5. Sato, K. et al. (1992) Tohoku J. Exp. Med. 168:97.

6. Townsend, D.M. and K.D. Tew (2003) Oncogene 22:7369.

7. Adler, V. et al. (1999) EMBO J. 18:1321.

• Entrez Gene IDs:

  2950 (Human)

• Alternate Names:

  deafness, X-linked 7; DFN7; EC 2.5.1.18; FAEES3; fatty acid ethyl ester synthase III; glutathione S-transferase P; Glutathione STransferase pi 1; Glutathione S-Transferase pi 1; GST class-pi; GST3DFN7; GSTP; GSTP1; GSTP1-1; PI