• Species Reactivity

  Human

• Specificity

  Detects human TBX3 in direct ELISAs and Western blots.  In direct ELISAs, less than 1% cross-reactivity with recombinant human (rh) TBX2, rhTBX6, and rhTBX20 is observed.    
   

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human TBX3    
  Ser321-Thr483    
  Accession # O15119

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

• Immunocytochemistry

  5-15 μg/mL

  Immersion fixed ZR-75-1 human mammary ductal carcinoma cell line

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: TBX3

TBX3 belongs to the T-box family of transcription factors, which are involved in regulation of developmental processes. TBX3 acts as a transcriptional repressor regulating anterior/posterior axis of the forelimb. Mutations in TBX3 are associated with ulnar-mammary syndrome, affecting limb, apocrine gland, tooth, hair, and genital development. TBX3 has 3 splice isoforms.

• Long Name:

  T-box Transcription Factor 3

• Entrez Gene IDs:

  6926 (Human); 21386 (Mouse); 353305 (Rat)

• Alternate Names:

  bladder cancer related protein XHL; T-box 3; T-box protein 3; T-box transcription factor TBX3; TBX3; TBX3-ISO; ulnar mammary syndrome; UMS; XHL