• Species Reactivity

  Human

• Specificity

  Detects endogenous human Miz-1 in Western blots.

• Source

  Polyclonal Goat IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  E. coli-derived recombinant human Miz-1    
  Met1-Ala188    
  Accession # Q13105

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Miz-1/ZBTB17

Miz-1 (Myc-interacting zinc finger protein 1; also ZBTB17) is an 88 kDa, DNA-binding protein that belongs to the Kruppel C2H2-type zinc finger protein family. Human Miz-1 is 803 amino acids (aa) in length, and contains a POZ domain (aa 1 - 104), two Myc-interaction segments (aa 269 - 308 & 637 - 718), and 12 C2H2-type zinc finger motifs (aa 306 - 637); also, there is one alternate splice form that shows an eight aa substitution for Thr769. Miz-1 induces cell arrest at G  ₁, an effect mediated by its activation of the gene coding for p15  ^INK4b. This effect is blocked by Myc, which displaces transcriptional coactivators bound to Miz-1. Notably, although the downregulation of Miz-1 may contribute to Myc-induced cell transformation, the deactivation of Miz-1 is absolutely essential for Myc-induced apoptosis. Over aa 1?188, human Miz-1 is 85% aa identical to mouse Miz-1.

• Long Name:

  Myc-interacting Zinc Finger Protein-1

• Entrez Gene IDs:

  7709 (Human); 22642 (Mouse); 313666 (Rat)

• Alternate Names:

  MIZ1; Miz-1; Myc-interacting zinc finger protein 1; mZ13; pHZ-67; ZBTB17; Zfp100; zinc finger and BTB domain containing 17; zinc finger and BTB domain-containing protein 17; zinc finger protein 151 (pHZ-67); Zinc finger protein 151; Zinc finger protein 60MIZ-1; ZNF151; ZNF151Myc-interacting Zn finger protein-1; ZNF60