• Species Reactivity

  Human

• Specificity

  Detects human Serpin A7/TBG in direct ELISAs and Western blots.  In direct ELISAs, approximately 5% cross-reactivity with recombinant human Serpin A9 is observed.

• Source

  Polyclonal Sheep IgG

• Purification

  Antigen Affinity-purified

• Immunogen

  Chinese hamster ovary cell line CHO-derived recombinant human Serpin A7/TBG    
  Ala21-Ala415    
  Accession # P05543

• Formulation

  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 μm filtered solution in PBS.

• Label

  Unconjugated

Applications

• Recommended    
  Concentration

  Sample

• Western Blot

  1 μg/mL

  See below

• 
  

Please Note: Optimal dilutions should be determined by each laboratory for each application.  are available in the Technical Information section on our website.

Data Examples

Preparation and Storage

• Reconstitution

  Reconstitute at 0.2 mg/mL in sterile PBS.

• Shipping

  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

• Stability & Storage

  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.    

• 12 months from date of receipt, -20 to -70 °C as supplied.

• 1 month, 2 to 8 °C under sterile conditions after reconstitution.

• 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Serpin A7/TBG

Serpin A7 (also TBG/Thyroxine-binding globulin and T4-binding globulin) is a secreted 54 kDa glycoprotein, non-inhibitory member of the serpin family of proteins. It is expressed by the liver and secreted in plasma. Mutations in the SERPINA7 gene can result in a whole spectrum of deficiencies, characterized by either reduced or increased TBG levels in the serum. A deficiency in TBG result in low serum total T4 and T3 count in clinically euthyroid subjects in the presence of normal serum TSH. Over aa21-415, human serpin A7 shares 76% aa sequence identity with mouse serpin A7.        

• Entrez Gene IDs:

  6906 (Human); 331535 (Mouse); 81806 (Rat)

• Alternate Names:

  antitrypsin), member 7; FLJ17820; member 7; Serpin A7; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); T4-binding globulin; TBG; TBGclade A (alpha-1 antiproteinase; thyroxin-binding globulin; thyroxine-binding globulin