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詳細(xì)說明
概述
產(chǎn)品名稱Anti-LAMP2抗體[EPR19531]
描述
兔單克隆抗體[EPR19531] to LAMP2
經(jīng)測(cè)試應(yīng)用IHC-P,WB
種屬反應(yīng)性
與反應(yīng): Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human LAMP2 aa 50-150. The exact sequence is proprietary.
Database link: P13473陽性對(duì)照
WB: Human fetal kidney, fetal spleen and placenta lysates; HeLa, THP-1, JAR and Jurkat whole cell lysates. IHC-P: Human liver and breast cancer tissues.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam's RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5, 675, 063 and/or 7, 429, 487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲(chǔ)溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號(hào)EPR19531
同種型IgG
研究領(lǐng)域
Cancer
Cell Death
Autophagy
Autophagosome
Metabolism
Pathways and Processes
Metabolism processes
Autophagy and mitophagy
Autophagosome
Metabolism
Pathways and Processes
Metabolic signaling pathways
Energy transfer pathways
Energy Metabolism
Stem Cells
Hematopoietic Progenitors
Myeloid
Monocytic Lineage
Stem Cells
Hematopoietic Progenitors
Myeloid
Myeloid Progenitor
Cardiovascular
Heart
Autophagy
Autophagosome
Immunology
Secreted Molecules
Other secreted molecules
Signal Transduction
Metabolism
Energy Metabolism
Tags & Cell Markers
Subcellular Markers
Organelles
Lysosome
Anti-LAMP2 antibody [EPR19531] 圖像
![Western blot - Anti-LAMP2 antibody [EPR19531] (ab199947)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199947WBa.jpg)
Western blot - Anti-LAMP2 antibody [EPR19531] (ab199947)
All lanes : Anti-LAMP2 antibody [EPR19531] (ab199947) at 1/1000 dilution
Lane 1 : Human fetal kidney lysate
Lane 2 : Human fetal spleen lysate
Lane 3 : Human placenta lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG Peroxidase Conjugate, specific to the non-reduced form of IgG at 1/100000 dilution
Predicted band size : 45 kDa
Observed band size : 110-130 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 19828315) and lots of other products.
![Western blot - Anti-LAMP2 antibody [EPR19531] (ab199947)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199947WBb.jpg)
Western blot - Anti-LAMP2 antibody [EPR19531] (ab199947)
All lanes : Anti-LAMP2 antibody [EPR19531] (ab199947) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : THP-1 (Human monocytic leukemia cell line) whole cell lysate
Lane 3 : JAR (Human placenta choriocarcinoma cell line) whole cell lysate
Lane 4 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 20 μg per lane.
Secondary
Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/100000 dilution
Predicted band size : 45 kDa
Observed band size : 110-130 kDa (why is the actual band size different from the predicted?)
Exposure time : 3 minutesBlocking/Dilution buffer: 5% NFDM/TBST.
The molecular weight observed is consistent with what has been described in the literature (PMID: 19828315) and lots of other products.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR19531] (ab199947)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199947HCa.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR19531] (ab199947)
Immunohistochemical analysis of paraffin-embedded Human liver tissue labeling LAMP2 with ab199947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
Cytoplasm staining on Human liver is observed.
Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR19531] (ab199947)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab19/ab199947HCb.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-LAMP2 antibody [EPR19531] (ab199947)
Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue labeling LAMP2 with ab199947 at 1/2000 dilution, followed by Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution. Cytoplasm staining on Human breast cancer is observed. Counter stained with Hematoxylin.
Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody is Goat Anti-Rabbit IgG H&L (HRP) (ab97051) at 1/500 dilution.
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