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詳細說明
概述
產品名稱Anti-Methionyl Aminopeptidase 1抗體[EPR15452(B)]
描述
兔單克隆抗體[EPR15452(B)] to Methionyl Aminopeptidase 1
經測試應用WB,IHC-P,ICC/IF,Flow Cyt
種屬反應性
與反應: Mouse, Rat, Human
免疫原
Synthetic peptide (the amino acid sequence is considered to be commercially sensitive) within Human Methionyl Aminopeptidase 1 aa 100-200. The exact sequence is proprietary.
Database link: P53582陽性對照
A375 and HUVEC lysate. Human cervix carcinoma and Rat skeletal muscle tissue. A549 and A375 cells.
常規(guī)說明
This product is a recombinant rabbit monoclonal antibody.
Produced using Abcam’s RabMAb? technology. RabMAb? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.
性能
形式Liquid
存放說明Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
存儲溶液Preservative: 0.01% Sodium azide
Constituents: 59% PBS, 40% Glycerol, 0.05% BSA純度Protein A purified
克隆單克隆
克隆編號EPR15452(B)
同種型IgG
研究領域
Metabolism
Pathways and Processes
Metabolic signaling pathways
Amino acid metabolism
Signal Transduction
Metabolism
Amino Acids
Anti-Methionyl Aminopeptidase 1 antibody [EPR15452(B)] 圖像
![Flow Cytometry - Anti-Methio<i></i>nyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185540406.jpg)
Flow Cytometry - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
Flow cytometry analysis of 2% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/180 dilution (pink) or a rabbit IgG isotype control (green), followed by secondary Goat anti rabbit IgG (FITC) at 1/150 dilution.
![Immunocytochemistry/ Immunofluorescence - Anti-Methio<i></i>nyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185540404.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
Immunofluorescent analysis of 4% paraformaldehyde fixed A375 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor?488) at a 1/200 dilution. Counterstained with Dapi blue.
![Immunocytochemistry/ Immunofluorescence - Anti-Methio<i></i>nyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185540403.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
Immunofluorescent analysis of 4% paraformaldehyde fixed A549 cells labeling Methionyl Aminopeptidase 1 with ab185540 at 1/250 dilution. Secondary antibody Goat anti rabbit IgG (Alexa Fluor?555) at a 1/200 dilution. Counterstained with Dapi blue.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methio<i></i>nyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185540540.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
Immunohistochemical analysis of paraffin-embedded Rat skeletal muscle tissue labeling Methionyl Aminopeptidase 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.
![Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methio<i></i>nyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)](http://img.lianshimall.com/statics/attachment/goods/pl20160426/abcamMainImgPrimary/detail/ab18/ab185540402.jpg)
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
Immunohistochemical analysis of paraffin-embedded Human cervix carcinoma tissue labeling Methionyl Aminopeptidase 1 with ab185540 at 1/100 dilution. Counter stain Hematoxylin.
Western blot - Anti-Methionyl Aminopeptidase 1 [EPR15452(B)] antibody (ab185540)
All lanes : Anti-Methionyl Aminopeptidase 1 antibody [EPR15452(B)] (ab185540) at 1/1000 dilution
Lane 1 : A375 cell lysate
Lane 2 : HUVEC cell lysate
Lysates/proteins at 10 μg per lane.
Secondary
Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/1000 dilution
Predicted band size : 43 kDa
Observed band size : 43 kDa
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