概述

• 產(chǎn)品名稱(chēng)Anti-MLH1抗體[EPR3893]
     

• 描述

  兔單克隆抗體[EPR3893] to MLH1

• 經(jīng)測(cè)試應(yīng)用WB

• 種屬反應(yīng)性

  與反應(yīng): Mouse, Rat, Human    
   

• 免疫原

  Synthetic peptide (the amino acid sequence is considered to be commercially sensitive)

• 陽(yáng)性對(duì)照    

• 293T, Jurkat, K562 and SH-SY5Y cell lysates

• 常規(guī)說(shuō)明

  This product is a recombinant rabbit monoclonal antibody.

  Produced using Abcam’s RabMAb^? technology. RabMAb^? technology is covered by the following U.S. Patents, No. 5,675,063 and/or 7,429,487.

性能

• 形式Liquid

• 存放說(shuō)明Shipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.

• 存儲(chǔ)溶液PBS 49%,Sodium azide 0.01%,Glycerol 50%,BSA 0.05%

• 純度Tissue culture supernatant

• 克隆單克隆

• 克隆編號(hào)EPR3893

• 同種型IgG

• 研究領(lǐng)域

• Cancer

• Oncoproteins/suppressors

• Tumor suppressors

• Other

• <a javascript:void(0)>Epigenetics and Nuclear Signaling

• DNA / RNA

• DNA Damage & Repair

• Mismatch Repair

Anti-MLH1 antibody [EPR3893] 圖像

• 

  Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)          

     
  
  Predicted band size : 85 kDa
     

  Lane 1: Wild-type HAP1 cell lysate (20 μg)
  Lane 2: MLH1 knockout HAP1 cell lysate (20 μg)
  Lane 3: HCT116 cell lysate (20 μg)
  Lane 4: 293T cell lysate (20 μg)
  Lanes 1 - 4: Merged signal (red and green). Green - ab108622 observed at 88 kDa. Red - loading control, ab8245, observed at 37 kDa.
  ab108622 was shown to recognize MLH1 when MLH1 knockout samples were used, along with additional cross-reactive bands. Wild-type and MLH1 knockout samples were subjected to SDS-PAGE. ab108622 and ab8245 (loading control to GAPDH) were diluted 1/1000 and 1/2000 and incubated overnight at 4°C. Blots were developed with goat anti-rabbit IgG (H + L) and goat anti-mouse IgG (H + L) secondary antibodies at 1/10 000 dilution for 1 h at room temperature before imaging.

   

• 

  Western blot - MLH1 antibody [EPR3893] (ab108622)          

     All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
  
  Lane 1 : 293T cell lysate
  Lane 2 : Jurkat cell lysate
  Lane 3 : K562 cell lysate
  Lane 4 : SH-SY5Y cell lysate
  
  Lysates/proteins at 10 μg per lane.
  
  
  Predicted band size : 85 kDa
   

• 

  Western blot - Anti-MLH1 antibody [EPR3893] (ab108622)      Image courtesy anonymous abreview    

     All lanes : Anti-MLH1 antibody [EPR3893] (ab108622) at 1/1000 dilution
  
  Lane 1 : Hela Cells Whole Cell Lysates
  Lane 2 : HCT116 Whole Cell Lysates
  
  Lysates/proteins at 30 μg per lane.
  
  Secondary
  Goat Polyclonal to Rabbit IgG (HRP) at 1/2000 dilution
  developed using the ECL technique
  
  Performed under reducing conditions.
  
  Predicted band size : 85 kDa
  
  
  Exposure time : 30 seconds

  Image courtesy anonymous abreview

     

  See Abreview