生物來(lái)源

human blood

種族

Caucasian

性別

female

生長(zhǎng)模式

Suspension

核型

Not specified

形態(tài)學(xué)

Lymphoblastoid

應(yīng)用

cell culture | mammalian: suitable

運(yùn)輸

dry ice

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相關(guān)類(lèi)別

Mammalian Cell Lines

細(xì)胞系描述

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC).  HLA-Typing Data is available for this cell line.

DNA圖譜分析

Not specified

血清學(xué)分析

HLA A: 1 3
HLA C: 7 0
HLA B: 7 8
HLA DR: 2 4
HLA DQ: 1 3

培養(yǎng)基

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442).  Once a growing culture is established the FBS concentration can be reduced to 10%.

傳代培養(yǎng)常規(guī)

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C.  Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

其他說(shuō)明

Additional freight & handling charges may be applicable for Asia-Pacific shipments.  Please check with your local Customer Service representative for more information.

免責(zé)聲明

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.