詳細(xì)說(shuō)明
生物來(lái)源
human blood
種族
Caucasian
性別
female
生長(zhǎng)模式
Suspension
核型
Not specified
形態(tài)學(xué)
Lymphoblastoid
應(yīng)用
cell culture | mammalian: suitable
運(yùn)輸
dry ice
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相關(guān)類(lèi)別
Mammalian Cell Lines
細(xì)胞系描述
This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.
DNA圖譜分析
Not specified
血清學(xué)分析
HLA A: 1 3
HLA C: 7 0
HLA B: 7 8
HLA DR: 2 4
HLA DQ: 1 3
培養(yǎng)基
RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.
傳代培養(yǎng)常規(guī)
Maintain cultures between 3x105 - 2x106 cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.
其他說(shuō)明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
免責(zé)聲明
This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.